Purification and properties of the catalytic domain of the thermostable pullulanase type II from Thermococcus hydrothermalis

被引：25

作者：

Erra-Pujada, M ^{[1
]}

Chang-Pi-Hin, F ^{[1
]}

Debeire, P ^{[1
]}

Duchiron, F ^{[1
]}

O'Donohue, MJ ^{[1
]}

机构：

[1] Ctr Rech Agron, UMR FARE 614, Inst Natl Rech Agron, F-51686 Reims 02, France

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 16期

关键词：

glycosidase; hyperthermostable; pullulanase type II; Thermococcales;

D O I：

10.1023/A:1010597619811

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A pullulanase type II was produced in Escherichia coli using the relevant gene from Thermococcus hydrothermalis. This protein was purified and its pullulanolytic and amylolytic activities were characterised. The optimum temperature and Ca2+ concentration for each activity were identical (105 degreesC and 0.09 mM), whereas the optimum pH (pH(pullulan) 5.75, pH(amylose) 5) and the influence of Ca2+ ions on the kinetic parameters were different. Further analyses revealed that this enzyme exhibits an endo-processive-like action and specifically cleaves alpha -1,6 bonds in pullulan.

引用

页码：1273 / 1277

页数：5

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