Characterization of Pasteurella multocida isolates from wetland ecosystems during 1996 to 1999

被引:8
作者
Samuel, MD
Shadduck, DJ
Goldberg, DR
Wilson, MA
Joly, DO
Lehr, MA
机构
[1] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI 53711 USA
[2] Wisconsin Cooperat Wildlife Res Unit, US Geol Survey, Dept Wildlife Ecol, Russell Labs 204, Madison, WI 53706 USA
[3] USDA, Anim & Plant Hlth Inspect Serv, Vet Serv, Natl Vet Serv Labs, Ames, IA 50010 USA
[4] Univ Vermont, Dept Biol, Burlington, VT 05405 USA
关键词
avian cholera; DNA fingerprinting; Pasteurella multocida; serotyping; wetlands;
D O I
10.7589/0090-3558-39.4.798
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
We cultured 126 Pasteurella multocida isolates, 92 from water and 34 from sediment samples collected from wetlands in the Pacific and Central flyways of the United States between 1996 and 1999. Most (121) of the isolates were P. multocida serotype 1, but serotypes 3, 3/4, 10, and 11 were also found. Many (82) of the isolates were further characterized by DNA fingerprinting procedures and tested in Pekin ducks for virulence. Almost all the serotype I isolates we tested caused mortality in Pekin ducks. Serotype I isolates varied in virulence, but the most consistent pattern was higher mortality in male ducks than in females. We found no evidence that isolates found in sediment vs. water, between Pacific and Central flyways, or during El Nino years had consistently different virulence. We also found a number of non-serotype I isolates that were avirulent in Pekin ducks. isolates had DNA fingerprint profiles similar to those found in birds that died during avian cholera outbreaks.
引用
收藏
页码:798 / 807
页数:10
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