Characterization of oligodeoxyribonucleotide-polyethylene glycol conjugates by electrospray mass spectrometry

被引：19

作者：

Tarasow, TM

Tinnermeier, D

Zyzniewski, C

机构：

[1] NeXstar Pharmaceuticals Inc., Boulder, CO 80301

来源：

BIOCONJUGATE CHEMISTRY | 1997年 / 8卷 / 01期

关键词：

D O I：

10.1021/bc960082+

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrospray mass spectrometry (ESMS) was used to characterize a number of differently functionalized polyethylene glycol-oligodeoxyribonucleotide conjugates. Sample preparation was found to be crucial to obtaining quality data. The resolution and precision of ESMS allowed for the identification of individual conjugates differing in MW by a single ethylene glycol unit with an accuracy of less than or equal to 1 (0.02% of the molecular weight). In addition, ESMS was shown to be valuable in identifying chromatographically unresolvable components of a derivatized polyethylene glycol-oligonucleotide conjugate mixture.

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页码：89 / 93

页数：5

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