Pol III binding in six mammals shows conservation among amino acid isotypes despite divergence among tRNA genes

被引：83

作者：

Kutter, Claudia ^{[1
,2
]}

Brown, Gordon D. ^{[1
]}

Goncalves, Angela ^{[3
,4
]}

Wilson, Michael D. ^{[1
,2
]}

Watt, Stephen ^{[1
]}

Brazma, Alvis ^{[3
]}

White, Robert J. ^{[5
]}

Odom, Duncan T. ^{[1
,2
,6
]}

机构：

[1] Canc Res UK, Cambridge Res Inst, Li Ka Shing Ctr, Cambridge, England

[2] Univ Cambridge, Dept Oncol, Hutchison Med Res Council Res Ctr, Cambridge, England

[3] European Bioinformat Inst, European Mol Biol Lab, Hinxton, England

[4] Univ Cambridge, Grad Sch Life Sci, Cambridge, England

[5] Beatson Inst Canc Res, Glasgow, Lanark, Scotland

[6] Wellcome Trust Sanger Inst, Cambridge, England

来源：

NATURE GENETICS | 2011年 / 43卷 / 10期

基金：

欧洲研究理事会; 瑞士国家科学基金会;

关键词：

TRANSCRIPTION FACTOR-BINDING; POLYMERASE-III; TRANSPOSABLE ELEMENTS; GENOME SEQUENCE; NONCODING RNAS; CODON USAGE; HUMAN-CELLS; STEM-CELLS; EXPRESSION; EVOLUTION;

D O I：

10.1038/ng.906

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

RNA polymerase III (Pol III) transcription of tRNA genes is essential for generating the tRNA adaptor molecules that link genetic sequence and protein translation. By mapping Pol III occupancy genome-wide in mouse, rat, human, macaque, dog and opossum livers, we found that Pol III binding to individual tRNA genes varies substantially in strength and location. However, when we took into account tRNA redundancies by grouping Pol III occupancy into 46 anticodon isoacceptor families or 21 amino acid-based isotype classes, we discovered strong conservation. Similarly, Pol III occupancy of amino acid isotypes is almost invariant among transcriptionally and evolutionarily diverse tissues in mouse. Thus, synthesis of functional tRNA isotypes has been highly constrained, although the usage of individual tRNA genes has evolved rapidly.

引用

页码：948 / U50

页数：10

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