Specificity of IS6110-based DNA fingerprinting and diagnostic techniques for Mycobacterium tuberculosis complex

被引：16

作者：

Githui, WA

Wilson, SM

Drobniewski, FA

机构：

[1] Univ London Kings Coll, Sch Med & Dent,Kings Coll Hosp Dulwich, PHLS Mycobactierium Reference Unit, Dulwich Publ Hth Lab, London SE22 8QF, England

[2] Univ London Kings Coll, Sch Med & Dent, Dulwich Hosp,Dept Microbiol, London SE22 8PT, England

[3] Univ London London Sch Hyg & Trop Med, Dept Infect & trop Dis, London, England

[4] Kenya Med Res Inst, Ctr Resp Dis Res, Nairobi, Kenya

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 04期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/JCM.37.4.1224-1226.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific primers yielded a 181-bp fragment only in DNA from M. tuberculosis complex isolates.

引用

页码：1224 / 1226

页数：3

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