Characterization and regulation of NADP+-isocitrate dehydrogenase from Saccharopolyspora erythraea

NADP(+)-Isocitrate dehydrogenase (ICDH) activity was detected in cell-free extracts of Saccharopolyspora erythraea CA340, an erythromycin producer. Apparent K-m values for DL-isocitrate and NADP(+) were 0.14 muM and 0.026 muM, respectively. ATP, ADP, GTP, citric acid, oxaloacetate, alpha-ketoglutarate, glyoxalate and glyoxalate plus oxaloacetate, each at 1 mM concentration, caused 50, 20 10, 50, 25, 60, 20 and 50% inhibition of ICDH activity, respectively. Phosphoenolpyruvate, fructose 1,6-diphosphate and pyruvate had no effect. ICDH specific activity profile was growth-associated and activity with dextrose or fructose as sole carbon source, was twice of that obtained with lactose.