Inhibitory activity of berberine on DNA strand cleavage induced by hydrogen peroxide and cytochrome c

被引：37

作者：

Choi, DS

Kim, SJ

Jung, MY

机构：

[1] Woosuk Univ, Div Food Sci & Technol, Wanju Jun 565701, Jeonbuk Provinc, South Korea

[2] Woosuk Univ, Div Biosci & Biotechnol, Wanju Jun 565701, Jeonbuk Provinc, South Korea

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2001年 / 65卷 / 02期

关键词：

berberine; DNA strand cleavage; hydrogen peroxide; cytochrome c; superoxide anion;

D O I：

10.1271/bbb.65.452

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The inhibitory activity of berberine on the DNA single-strand cleavage induced by hydrogen peroxide and cytochrome c was measured. Berberine effectively inhibited single-strand cleavage of DNA and its effectiveness was concentration-dependent. As the berberine concentration increased, the inhibitory activity against the DNA single-strand cleavage increased. The treatments with 1, 5, 10, 50, and 100 muM berberine showed 7.7, 10.8, 32.2, 39.5, and 51.6% inhibition of DNA cleavage. This inhibitory activity of berberine against the DNA single-strand cleavage has never been reported previously. The inhibitory activity of berberine against DNA cleavage was stronger than caffeic acid and ascorbic acid. Berberine did not show strong hydroxyl radical scavenging activity, but showed strong superoxide anion radical quenching ability.

引用

页码：452 / 455

页数：4

共 12 条

[1] Effects of berberine on arylamine N-acetyltransferase activity in human bladder tumour cells [J].