Sensitization of cells and retroviruses to human serum by (alpha 1-3) galactosyltransferase

被引：237

作者：

Takeuchi, Y ^{[1
]}

Porter, CD ^{[1
]}

Strahan, KM ^{[1
]}

Preece, AF ^{[1
]}

Gustafsson, K ^{[1
]}

Cosset, FL ^{[1
]}

Weiss, RA ^{[1
]}

Collins, MKL ^{[1
]}

机构：

[1] UNIV LONDON,INST CHILD HLTH,LONDON WC1N 1EH,ENGLAND

来源：

NATURE | 1996年 / 379卷 / 6560期

关键词：

D O I：

10.1038/379085a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

MAMMALIAN C-type retroviruses are inactivated by human serum(1,2), following triggering of the classical complement cascade(3). This may have inhibited transmission to humans of C-type oncoviruses from other mammals(1). Indeed, the retroviruses human immunodeficiency virus and human T-cell leukaemia virus are resistant of human C1q, the first component of the classical pathway, by retroviral envelope proteins has been described(6). However, retroviruses produced from human cells are resistant to inactivation by human complement(7,8) and human serum is known to contain antibodies directed against carbohydrates on retroviral envelopes(9-11). Gal(alpha 1-3)Gal terminal carbohydrates are expressed by most mammals but are absent in humans, which lack a functional (alpha 1-3)galactosyltransferase gene (12,13). Here, we demonstrate that anti-Gal(alpha 1-3)Gal antibodies in human serum inactivate retroviruses produced from animal cells. Expression of porcine (alpha 1-3)galactosyltransferase(14,16) in human cells renders the cells and the retroviruses they produce sensitive to human serum.

引用

页码：85 / 88

页数：4

共 30 条

[11] GENE-SEQUENCES SUGGEST INACTIVATION OF ALPHA-1,3-GALACTOSYLTRANSFERASE IN CATARRHINES AFTER THE DIVERGENCE OF APES FROM MONKEYS [J].