Synaptophysin Regulates the Kinetics of Synaptic Vesicle Endocytosis in Central Neurons

被引：389

作者：

Kwon, Sung E. ^{[1
,2
]}

Chapman, Edwin R. ^{[1
,2
]}

机构：

[1] Univ Wisconsin, Howard Hughes Med Inst, Madison, WI 53706 USA

[2] Univ Wisconsin, Dept Neurosci, Madison, WI 53706 USA

来源：

NEURON | 2011年 / 70卷 / 05期

关键词：

TYROSINE PHOSPHORYLATION; HIPPOCAMPAL-NEURONS; CHANNEL PROTEIN; SYNAPTOTAGMIN-I; MEMBRANE; SYNAPSES; IDENTIFICATION; SYNAPTOBREVIN; BIOGENESIS; MECHANISM;

D O I：

10.1016/j.neuron.2011.04.001

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Despite being the most abundant synaptic vesicle membrane protein, the function of synaptophysin remains enigmatic. For example, synaptic transmission was reported to be completely normal in synaptophysin knockout mice; however, direct experiments to monitor the synaptic vesicle cycle have not been carried out. Here, using optical imaging and electrophysiological experiments, we demonstrate that synaptophysin is required for kinetically efficient endocytosis of synaptic vesicles in cultured hippocampal neurons. Truncation analysis revealed that distinct structural elements of synaptophysin differentially regulate vesicle retrieval during and after stimulation. Thus, synaptophysin regulates at least two phases of endocytosis to ensure vesicle availability during and after sustained neuronal activity.

引用

页码：847 / 854

页数：8

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