PCR analysis of cry7 genes in Bacillus thuringiensis by the five conserved blocks of toxins

被引：10

作者：

Ben-Dov, E ^{[1
]}

Manasherob, R ^{[1
]}

Zaritsky, A ^{[1
]}

Barak, Z ^{[1
]}

Margalith, Y ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel

来源：

CURRENT MICROBIOLOGY | 2001年 / 42卷 / 02期

关键词：

D O I：

10.1007/s0028403336

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An alternative PCR analysis to screen for cry7 genes is proposed, based on the five conserved blocks of amino acids of Bacillus thuringiensis toxins and their encoding DNA sequences. A complete set of five primers was constructed, four direct and one reverse, yielding four specific amplicons. Modified profiles can identify new cry genes.

引用

收藏

页码：96 / 99

页数：4

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