Lipopolysaccharide-stimulated activation of murine DC2.4 cells is attenuated by n-butylidenephthalide through suppression of the NF-κB pathway

被引：42

作者：

Fu, Ru-Huei ^{[1
,2
]}

Hran, Horng-Jyh ^{[3
]}

Chu, Ching-Liang ^{[2
,4
]}

Huang, Chin-Mao ^{[2
]}

Liu, Shih-Ping ^{[1
,5
]}

Wang, Yu-Chi ^{[6
]}

Lin, Ya-Hsien ^{[2
]}

Shyu, Woei-Cherng ^{[1
,2
]}

Lin, Shinn-Zong ^{[1
,2
,7
]}

机构：

[1] China Med Univ Hosp, Ctr Neuropsychiat, Taichung 40402, Taiwan

[2] China Med Univ, Grad Inst Immunol, Taichung 40402, Taiwan

[3] China Med Univ Hosp, Dept Pathol, Taichung 40402, Taiwan

[4] Natl Hlth Res Inst, Immunol Res Ctr, Zhunan 350, Miaoli, Taiwan

[5] China Med Univ, Grad Inst Basic Med Sci, Taichung 40402, Taiwan

[6] Biotechplex Corp, San Marcos, CA 92078 USA

[7] China Med Univ, Beigang Hosp, Beigang 65152, Yunlin, Taiwan

来源：

BIOTECHNOLOGY LETTERS | 2011年 / 33卷 / 05期

关键词：

Angelica sinensis; n-Butylidenephthalide; Dendritic cells; Immunomodulation; Nuclear factor-kappa B; ANGELICA-SINENSIS; GENE-EXPRESSION; DENDRITIC CELLS; HERBAL MEDICINE; RECEPTOR;

D O I：

10.1007/s10529-011-0528-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Modulation of dendritic cell (DC) fate and function may be one approach for the treatment of inflammatory and autoimmune diseases. n-Butylidenephthalide (BP), derived from Angelica sinensis, at 40 mu g/ml significantly decreased the secretion of interleukin-6 and tumor necrosis factor-alpha by lipopolysaccharide (LPS)-stimulated activation of cultured murine DC2.4 cells (P < 0.01). LPS-induced major histocompatibility complex class II (P < 0.05), CD86 (P < 0.01) and CD40 (P < 0.01) expression on DC2.4 cells was also inhibited by BP. The endocytic capacity of LPS-stimulated DC2.4 cells was increased by BP (P < 0.01). The antigen-presenting capacity of LPS-stimulated DC2.4 cells was decreased by BP (P < 0.05). Moreover, we confirmed BP attenuates the responses of LPS-stimulated activation of DCs via suppression of NF-kappa B-dependent pathways.

引用

页码：903 / 910

页数：8

共 23 条

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