Apoptosis induced by ischemia-reperfusion and fasting in gastric mucosa compared to small intestinal mucosa in rats

被引：32

作者：

Fukuyama, K ^{[1
]}

Iwakiri, R ^{[1
]}

Noda, T ^{[1
]}

Kojima, M ^{[1
]}

Utsumi, H ^{[1
]}

Tsunada, S ^{[1
]}

Sakata, H ^{[1
]}

Ootani, A ^{[1
]}

Fujimoto, K ^{[1
]}

机构：

[1] Saga Med Sch, Dept Internal Med, Saga 8498501, Japan

来源：

DIGESTIVE DISEASES AND SCIENCES | 2001年 / 46卷 / 03期

关键词：

percent fragmented DNA; DNA ladder; TUNEL; programmed cell death;

D O I：

10.1023/A:1005695031233

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The effect of ischemia-reperfusion and 48-hr fasting on apoptosis was characterized in rat gastric mucosa and compared to small intestinal mucosa. Under halothane anesthesia, the celiac artery or superior mesenteric artery in the rat was occluded for 60 min followed by reperfusion. Occlusion of the celiac artery reduced blood flow in the stomach and occlusion of the mesenteric artery reduced blood flow in the small intestine. Additional rats were fasted for 48 hr to evaluate the effect of fasting on mucosal apoptosis. The ratios of fragmented DNA to total DNA, electrophoresis, and immunohistochemical staining were examined after ischemia-reperfusion or fasting. Apoptosis was not induced significantly in the gastric mucosa after ischemia-reperfusion, although it increased dramatically in the intestinal mucosa after ischemia-reperfusion. Further, after 48 fasting, apoptosis was induced in the small intestine, but not in the stomach. These results indicate that rat gastric mucosa is not as sensitive as small intestinal mucosa to ischemia-reperfusion or fasting-induced apoptosis.

引用

页码：545 / 549

页数：5

共 31 条

[1] TRIBUTYLTIN STIMULATES APOPTOSIS IN RAT THYMOCYTES [J].