Secondary structure and conserved motifs of the frequently sequenced domains IV and V of the insect mitochondrial large subunit rRNA gene

被引:118
作者
Buckley, TR [1 ]
Simon, C
Flook, PK
Misof, B
机构
[1] Duke Univ, Dept Zool, Durham, NC 27708 USA
[2] Victoria Univ Wellington, Sch Biol Sci, Inst Mol Systemat, Wellington, New Zealand
[3] Univ Connecticut, Dept Ecol & Evolutionary Biol, Storrs, CT 06269 USA
[4] Univ Basel, Inst Zool, CH-4003 Basel, Switzerland
[5] Univ Bonn, Inst Evolut Biol & Oekol, D-5300 Bonn, Germany
关键词
RNA secondary structure; alignment; molecular systematics; mitochondrial LSU rRNA; insect mitochondrial DNA;
D O I
10.1046/j.1365-2583.2000.00220.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have analysed over 400 partial insect mitochondrial large subunit (mit LSU) sequences in order to identify conserved motifs and secondary structures for domains IV and V of this gene. Most of the secondary structure elements described by R. R. Gutell et al. (unpublished) for the LSU were identified. However, we present structures for helices 84 and 91 that are not recognized in previous universal models. The portion of the 16S gene containing domains IV and V is frequently sequenced in insect molecular systematic studies so we have many more sequences than previous studies which focused on the complete mitochondrial LSU molecule. In addition, we have the advantage of investigating several sets of closely related taxa. Aligned sequences from thirteen insect orders and nine secondary structure diagrams are presented,These conserved sequence motifs and their associated secondary structure elements can now be used to facilitate the alignment of other insect mit LSU sequences.
引用
收藏
页码:565 / 580
页数:16
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