Evidence for the mechanism of photocatalytic degradation of the bacterial wall membrane at the TiO2 interface by ATR-FTIR and laser kinetic spectroscopy

被引:272
作者
Kiwi, J [1 ]
Nadtochenko, V [1 ]
机构
[1] Swiss Fed Inst Technol, Inst Chem Sci & Engn, Lab Photon & Interfaces, CH-1015 Lausanne, Switzerland
关键词
D O I
10.1021/la046983l
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The photocatalytic peroxidation of E. coli cell, lipo-polysaccharide (LPS), phosphatidyl-ethanolcholine (PE), and peptidoglycan (PGN) of the E. coli membrane wall has been investigated on TiO2 porous films by ATR-FTIR spectroscopy. The fast reactions of the photogenerated charge carriers in TiO2 with E. coli, LPS, and PE were monitored by laser kinetic spectroscopy. ATR-FTIR spectroscopy allowed the identification of E. coli, LPS, PE, and PGN as photocatalytic peroxidation products. The PGN was observed to be the most resistant membrane wall component. Shorter peroxidation times were observed for LPS and PE. Laser photolysis shows that E. coli, LPS, and PE compete in the scavenging of a surface trapped holes (h(+)) with the recombination reaction of h+ with the generated electrons (e(-)) within times > 50 ns. This scavenging leads to the formation of organic radicals initiating the radical chain peroxidation of E. coli, LPS, PE, and PE.
引用
收藏
页码:4631 / 4641
页数:11
相关论文
共 41 条
[1]   Conformational studies of synthetic lipid A analogues and partial structures by infrared spectroscopy [J].
Brandenburg, K ;
Kusumoto, S ;
Seydel, U .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1997, 1329 (01) :183-201
[2]   Physicochemical characteristics of triacyl lipid A partial structure OM-174 in relation to biological activity [J].
Brandenburg, K ;
Lindner, B ;
Schromm, A ;
Koch, MHJ ;
Bauer, J ;
Merkli, A ;
Zbaeren, C ;
Davies, JG ;
Seydel, U .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (11) :3370-3377
[3]  
CAMERON DG, 1986, SPECTROSC-BIOMED APP, P53
[4]   Photoelectrocatalytic and photocatalytic disinfection of E-coli suspensions by titanium dioxide [J].
Christensen, PA ;
Curtis, TP ;
Egerton, TA ;
Kosa, SAM ;
Tinlin, JR .
APPLIED CATALYSIS B-ENVIRONMENTAL, 2003, 41 (04) :371-386
[5]  
Colthup N.B., 1991, HDB INFRARED RAMAN C
[6]   Quantitative Fourier transform infrared analysis for anisidine value and aldehydes in thermally stressed oils [J].
Dubois, J ;
vandeVoort, FR ;
Sedman, J ;
Ismail, AA ;
Ramaswamy, HR .
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1996, 73 (06) :787-794
[7]  
Gué M, 2001, BIOCHEMISTRY-US, V40, P11938
[8]   IDENTIFICATION OF SOME BACTERIAL-CELL COMPONENTS BY FT-IR SPECTROSCOPY [J].
HELM, D ;
NAUMANN, D .
FEMS MICROBIOLOGY LETTERS, 1995, 126 (01) :75-79
[9]   Topology and transport of membrane lipids in bacteria [J].
Huijbregts, RPH ;
de Kroon, AIPM ;
de Kruijff, B .
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON BIOMEMBRANES, 2000, 1469 (01) :43-61
[10]  
Kacurakova M, 1996, CARBOHYD RES, V284, P145, DOI 10.1016/0008-6215(95)00412-2