Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

被引：180

作者：

Datta, K

Tu, JM

Oliva, N

Ona, I

Velazhahan, R

Mew, TW

Muthukrishnan, S

Datta, SK

机构：

[1] Int Rice Res Inst, Plant Breeding Genet & Biochem Div, Makati 1271, Philippines

[2] Int Rice Res Inst, Entomol & Plant Pathol Div, Makati 1271, Philippines

[3] Tamil Nadu Agr Univ, Dept Plant Pathol, Coimbatore, Tamil Nadu, India

[4] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA

来源：

PLANT SCIENCE | 2001年 / 160卷 / 03期

关键词：

sheath blight; chitinase; transgenic rice; enhanced resistance;

D O I：

10.1016/S0168-9452(00)00413-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Genetic transformation has been attempted for management of rice sheath blight disease, caused by Rhizoctonia solani. We introduced a PR-3 rice chitinase gene (RC7), isolated from R. solani-infected rice plants, into indica rice cultivars IR72, IR64, IR68899B, MH63, and Chinsurah Bore II by the biolistic and PEG-mediated transformation system. Inheritance was studied up to the T-2 generation by Southern blot analysis. Western blot analysis of transgenic plants with polyclonal antibody revealed the presence of chitinase protein with a molecular weight of 35 kDa that reacts with chitinase antibody. The transformants synthesized different levels of chitinase proteins constitutively and progeny from the plants containing the chitinase gene showed different levels of enhanced resistance when challenged with the sheath blight pathogen R. solani. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：405 / 414

页数：10

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