Expression of active barley seed ribosome-inactivating protein in transgenic wheat

被引：61

作者：

Bieri, S ^{[1
]}

Potrykus, I ^{[1
]}

Fütterer, J ^{[1
]}

机构：

[1] ETH Zurich, Inst Plant Sci, CH-8092 Zurich, Switzerland

来源：

THEORETICAL AND APPLIED GENETICS | 2000年 / 100卷 / 05期

关键词：

wheat transformation; ribosome-inactivating protein; anti-fungal protein; protein-targeting;

D O I：

10.1007/s001220051349

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Phenotypically normal, transgenic wheat (Triticum aestivum, var. Frisal) plants expressing a barley seed ribosome-inactivating protein (RIP) were produced. Expression was controlled by an intron-enhanced cauliflower mosaic virus 35S promoter and has been completely stable over four generations so far, possibly due to matrix-associated regions (MARs) that flank the transgenes. An engineered fusion to a signal peptide derived from the barley seed beta-1,3-glucanase caused the transport of RIP to the apoplast. Activity of the accumulated protein could be shown by significant inhibition of a rabbit reticulocyte transcription/translation system. Plants expressing high levels of RIP were protected only moderately or not at all against infection by the fungal pathogen Erysiphe graminis.

引用

页码：755 / 763

页数：9

共 50 条

[1] ISOLATION AND CHARACTERIZATION OF INHIBITORS OF ANIMAL CELL-FREE PROTEIN-SYNTHESIS FROM BARLEY-SEEDS [J].

ASANO, K ;

SVENSSON, B ;

POULSEN, FM .

CARLSBERG RESEARCH COMMUNICATIONS, 1984, 49 (07) :619-626

[2] CAULIFLOWER MOSAIC-VIRUS P35S PROMOTER ACTIVITY IN ESCHERICHIA-COLI [J].

ASSAAD, FF ;

SIGNER, ER .

MOLECULAR & GENERAL GENETICS, 1990, 223 (03) :517-520

[3] RIBOSOME-INACTIVATING PROTEINS FROM PLANTS [J].

BARBIERI, L ;

BATTELLI, MG ;

STIRPE, F .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1154 (3-4) :237-282

[4] FERTILE TRANSGENIC WHEAT FROM MICROPROJECTILE BOMBARDMENT OF SCUTELLAR TISSUE [J].

BECKER, D ;

BRETTSCHNEIDER, R ;

LORZ, H .

PLANT JOURNAL, 1994, 5 (02) :299-307

[5] Engineering changes in wheat flour by genetic transformation [J].

Blechl, AE ;

Le, HQ ;

Anderson, OD .

JOURNAL OF PLANT PHYSIOLOGY, 1998, 152 (06) :703-707

[6] Genetic engineering of wheat for increased resistance to powdery mildew disease [J].

Bliffeld, M ;

Mundy, J ;

Potrykus, I ;

Fütterer, J .

THEORETICAL AND APPLIED GENETICS, 1999, 98 (6-7) :1079-1086

[7] CHARACTERIZATION OF A PLANT SCAFFOLD ATTACHMENT REGION IN A DNA FRAGMENT THAT NORMALIZES TRANSGENE EXPRESSION IN TOBACCO [J].

BREYNE, P ;

VANMONTAGU, M ;

DEPICKER, A ;

GHEYSEN, G .

PLANT CELL, 1992, 4 (04) :463-471

[8] RICE TUNGRO BACILLIFORM VIRUS - TRANSCRIPTION AND TRANSLATION IN PROTOPLASTS [J].

CHEN, G ;

MULLER, M ;

POTRYKUS, I ;

HOHN, T ;

FUTTERER, J .

VIROLOGY, 1994, 204 (01) :91-100

[9] INHIBITORS OF ANIMAL CELL-FREE PROTEIN-SYNTHESIS FROM GRAINS [J].

COLEMAN, WH ;

ROBERTS, WK .

BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 696 (03) :239-244

[10] PROTEIN SECRETION IN PLANT-CELLS CAN OCCUR VIA A DEFAULT PATHWAY [J].

DENECKE, J ;

BOTTERMAN, J ;

DEBLAERE, R .

PLANT CELL, 1990, 2 (01) :51-59

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