Expression of active barley seed ribosome-inactivating protein in transgenic wheat

被引：61

作者：

Bieri, S ^{[1
]}

Potrykus, I ^{[1
]}

Fütterer, J ^{[1
]}

机构：

[1] ETH Zurich, Inst Plant Sci, CH-8092 Zurich, Switzerland

来源：

THEORETICAL AND APPLIED GENETICS | 2000年 / 100卷 / 05期

关键词：

wheat transformation; ribosome-inactivating protein; anti-fungal protein; protein-targeting;

D O I：

10.1007/s001220051349

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Phenotypically normal, transgenic wheat (Triticum aestivum, var. Frisal) plants expressing a barley seed ribosome-inactivating protein (RIP) were produced. Expression was controlled by an intron-enhanced cauliflower mosaic virus 35S promoter and has been completely stable over four generations so far, possibly due to matrix-associated regions (MARs) that flank the transgenes. An engineered fusion to a signal peptide derived from the barley seed beta-1,3-glucanase caused the transport of RIP to the apoplast. Activity of the accumulated protein could be shown by significant inhibition of a rabbit reticulocyte transcription/translation system. Plants expressing high levels of RIP were protected only moderately or not at all against infection by the fungal pathogen Erysiphe graminis.

引用

页码：755 / 763

页数：9

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