Assignment of gas-phase dipeptide amide hydrogen exchange rate constants by site-specific substitution: GlyGly

被引：20

作者：

He, F

Marshall, AG ^{[1
]}

Freitas, MA

机构：

[1] Florida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32310 USA

[2] Ohio State Univ, Columbus, OH 43210 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2001年 / 105卷 / 11期

关键词：

D O I：

10.1021/jp003492a

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Amide hydrogen deuterium exchange is widely used to probe conformations of gas-phase biomacromolecular ions. Meaningful interpretation of such experiments depends on relating the experimentally determined H/D exchange rate constants to specific exchangeable hydrogen sites on the molecule. Here, we present the first definitive assignment of H/D exchange rate constants for the N-terminal ammonium, amide hydrogen, and carboxyl hydrogens in diglycine, based on comparison of site-specific gas-phase H/D exchange rate constants for diglycine (GlyGly) and its selectively methylated homologues (GlySar, GlyGly-OMe, and GlySar-OMe). The present method may be extended to identification of site-specific H/D exchange rate constants for other dipeptides, to provide insight into H/D exchange reaction mechanisms.

引用

页码：2244 / 2249

页数：6

共 48 条

[1] PRINCIPLES THAT GOVERN FOLDING OF PROTEIN CHAINS [J].