Optimization of oligonucleotide arrays and RNA amplification protocols for analysis of transcript structure and alternative splicing

被引：65

作者：

Castle, J ^{[1
]}

Garrett-Engele, P ^{[1
]}

Armour, CD ^{[1
]}

Duenwald, SJ ^{[1
]}

Loerch, PM ^{[1
]}

Meyer, MR ^{[1
]}

Schadt, EE ^{[1
]}

Stoughton, R ^{[1
]}

Parrish, ML ^{[1
]}

Shoemaker, DD ^{[1
]}

Johnson, JM ^{[1
]}

机构：

[1] Merck & Co Inc, Rosetta Inpharmat, Kirkland, WA 98034 USA

来源：

GENOME BIOLOGY | 2003年 / 4卷 / 10期

关键词：

D O I：

10.1186/gb-2003-4-10-r66

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microarrays offer a high-resolution means for monitoring pre-mRNA splicing on a genomic scale. We have developed a novel, unbiased amplification protocol that permits labeling of entire transcripts. Also, hybridization conditions, probe characteristics, and analysis algorithms were optimized for detection of exons, exon-intron edges, and exon junctions. These optimized protocols can be used to detect small variations and isoform mixtures, map the tissue specificity of known human alternative isoforms, and provide a robust, scalable platform for high-throughput discovery of alternative splicing.

引用

页数：13

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