Characterization of a desiccation-related protein in lily pollen during development and stress

被引：53

作者：

Wang, CS ^{[1
]}

Liau, YE

Huang, JC

Wu, TD

Su, CC

Lin, CH

机构：

[1] Natl Chung Hsing Univ, Grad Inst Agr Biotechnol, Taichung 40227, Taiwan

[2] Natl Chung Hsing Univ, Agr Biotechnol Labs, Taichung 40227, Taiwan

[3] Natl Chung Hsing Univ, Dept Bot, Taichung 40227, Taiwan

来源：

PLANT AND CELL PHYSIOLOGY | 1998年 / 39卷 / 12期

关键词：

amino acid sequencing; desiccation; Lilium longiflorum; pollen protein; stress-induced;

D O I：

10.1093/oxfordjournals.pcp.a029335

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

This work characterizes a lily (Lilium longiflorum Thunb. cv, Snow Queen) anther (LLA) protein associated with desiccation. Peptide mapping analysis revealed that the abundant LLA-23 doublet contained similar polypeptides, having an isoelectric point of 6.1. Immunoblots of pollen protein from developing anther/pollen confirmed that the LLA-23 protein accumulated only at the later stage of pollen maturation and that the levels remained steady in mature and vital pollen. The accumulation of LLA-23 proteins was correlated with desiccation that naturally occurred in pollen. Subcellular fractionation of pollen proteins revealed that the protein was located in the cytoplasmic fraction. Premature drying of developing pollen confirmed that the concomitant accumulation of LLA-23 was associated with desiccation. Peptide sequence analysis demonstrates similarities between the lily LLA-23 and a family of water-deficit/ripening-induced proteins including LP3 of pine, DS2 of potato, and Asr of tomato and pummelo, In addition, the concomitant accumulation of LLA-23 can be experimentally manipulated by methyl jasmonate (MeJA) and salicylic acid (SA as well as by mannitol and methyl viologen. The LLA-23 represents a novel member of the water-deficit/ripenine-induced proteins.

引用

页码：1307 / 1314

页数：8

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