Subcellular localization of the small GTPase Rab5a in resting and stimulated human neutrophils

被引：24

作者：

Vita, F ^{[1
]}

Soranzo, MR ^{[1
]}

Borelli, V ^{[1
]}

Bertoncin, P ^{[1
]}

Zabucchi, G ^{[1
]}

机构：

[1] UNIV TRIESTE,IST PATOL GEN,I-34127 TRIESTE,ITALY

来源：

EXPERIMENTAL CELL RESEARCH | 1996年 / 227卷 / 02期

关键词：

D O I：

10.1006/excr.1996.0286

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The evidence that small GTPases of the Rab family are regulators of vesicle traffic which can influence various cell functions prompted us to investigate the potential role of one of these proteins, Rab5a, in human neutrophils. In this paper we show that a large amount of Rab5a is present in the cytosol of peripheral blood mature neutrophils. The remaining protein was found to be membrane and azurophilic granule associated. Upon neutrophil challenge with PMA for 10 min the amount of membrane-associated Rab5a was upregulated while the cytosolic content of the protein concomitantly decreased. These findings support the hypothesis that Rab5a could be involved in the mechanism of neutrophil activation by modulating the rate of endocytosis and/or vesicle fusion. (C) 1996 Academic Press, Inc.

引用

页码：367 / 373

页数：7

共 36 条

[11] THE INVOLVEMENT OF THE SMALL GTP-BINDING PROTEIN RAB5A IN NEURONAL ENDOCYTOSIS [J].