Secondary metabolites influence Arabidopsis/Botrytis interactions:: variation in host production and pathogen sensitivity

被引:217
作者
Kliebenstein, DJ
Rowe, HC
Denby, KJ
机构
[1] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA
[2] Univ Cape Town, Dept Mol & Cell Biol, ZA-7701 Rondebosch, South Africa
关键词
Arabidopsis thaliana; Botrytis cinerea; secondary metabolite; phytoalexin; virulence; spatial context;
D O I
10.1111/j.1365-313X.2005.02508.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Numerous studies have suggested that plant/pathogen interactions are partially mediated via plant secondary metabolite production and corresponding pathogen tolerance. However, there are inconsistent reports on the ability of particular compounds to provide resistance to a pathogen. Most of these studies have focused on individual isolates of a given pathogen, suggesting that pathogens vary in their sensitivity to plant-produced toxins. We tested variability in virulence among pathogen isolates, and the impact on this by plant production of, and pathogen tolerance to, secondary metabolites. Botrytis cinerea isolates showed differing sensitivity to purified camalexin, and camalexin-sensitive isolates produced larger lesions on camalexin-deficient Arabidopsis genotypes than on the wild type. In contrast, the camalexin-insensitive isolate produced lesions of similar size on wild-type and camalexin-deficient Arabidopsis. Additional analysis with Arabidopsis secondary metabolite biosynthetic mutants suggests that Botrytis also has variable sensitivity to phenylpropanoids and glucosinolates. Furthermore, Botrytis infection generates a gradient of secondary metabolite responses emanating from the developing lesion, with the Botrytis isolate used determining the accumulation pattern. Collectively, our results indicate that Arabidopsis/Botrytis interactions are influenced at the metabolic level by variations in toxin production in the host and sensitivity in the pathogen.
引用
收藏
页码:25 / 36
页数:12
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