Immunogenicity of Helicobacter pylori urease B protein and DNA vaccines in a mouse model

被引：6

作者：

Begue, Rodolfo E.

Cruz, Alma R.

Ramgoolam, Andres

Breslin, Mary B.

机构：

[1] Louisiana State Univ, Hlth Sci Ctr, Dept Pediat, New Orleans, LA USA

[2] Res Inst Childres, New Orleans, LA USA

来源：

JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION | 2007年 / 45卷 / 04期

关键词：

DNA vaccine; Helicobacter pylori; prime-boost approach;

D O I：

10.1097/MPG.0b013e31806c7bf8

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

A vaccine could alleviate major morbidity and mortality associated with Helicobacter pylori infection. We immunized BALB/c mice with 3 doses of a protein or DNA vaccine based on H pylori urease B. Protein alone was immunogenic even after the first dose, whereas DNA did not elicit antibodies after 3 doses. DNA preceding protein (D-P-P) appeared to blunt the response to protein, whereas DNA following protein (P-D-D) shifted from a predominantly T helper 2 (Th2) profile to a balanced Th1:Th2 profile. These preliminary findings may have important implications for the development of an H pylori vaccine. JPGN 45:493-496, 2007.

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页码：493 / 496

页数：4

相关论文

共 18 条

[1] Avidity of IgG for Streptococcus pneumoniae type 6B and 23F polysaccharides in infants primed with pneumococcal conjugates and boosted with polysaccharide or conjugate vaccines [J].

Anttila, M ;

Eskola, J ;

Åhman, H ;

Käyhty, H .

JOURNAL OF INFECTIOUS DISEASES, 1998, 177 (06) :1614-1621

[2] Immunology of Helicobacter pylori and prospects for vaccine [J].

Blanchard, TG ;

Czinn, SJ .

GASTROENTEROLOGY CLINICS OF NORTH AMERICA, 2000, 29 (03) :671-+

[3]

Dzwonek A, 2004, CELL MOL BIOL LETT, V9, P483

[4] ESSENTIAL ROLE OF UREASE IN PATHOGENESIS OF GASTRITIS INDUCED BY HELICOBACTER-PYLORI IN GNOTOBIOTIC PIGLETS [J].

EATON, KA ;

BROOKS, CL ;

MORGAN, DR ;

KRAKOWKA, S .

INFECTION AND IMMUNITY, 1991, 59 (07) :2470-2475

[5] Immunization of mice with urease vaccine affords protection against Helicobacter pylori infection in the absence of antibodies and is mediated by MHC class II-restricted responses [J].

Ermak, TH ;

Giannasca, PJ ;

Nichols, R ;

Myers, GA ;

Nedrud, J ;

Weltzin, R ;

Lee, CK ;

Kleanthous, H ;

Monath, TP .

JOURNAL OF EXPERIMENTAL MEDICINE, 1998, 188 (12) :2277-2288

[6] RECOMBINANT ANTIGENS PREPARED FROM THE UREASE SUBUNITS OF HELICOBACTER SPP - EVIDENCE OF PROTECTION IN A MOUSE MODEL OF GASTRIC INFECTION [J].

FERRERO, RL ;

THIBERGE, JM ;

HUERRE, M ;

LABIGNE, A .

INFECTION AND IMMUNITY, 1994, 62 (11) :4981-4989

[7] Systemic immunization with urease protects mice against Helicobacter pylori infection [J].

Guy, B ;

Hessler, C ;

Fourage, S ;

Haensler, J ;

Vialon-Lafay, E ;

Rokbi, B ;

Millet, MJQ .

VACCINE, 1998, 16 (08) :850-856

[8] A plasmid immunization construct encoding urease B of Helicobacter pylori induces an antigen-specific antibody response and upregulates the expression of β-defensins and IL-10 in the stomachs of immunized mice [J].

Hatzifoti, C ;

Bajaj-Elliott, M ;

Dorrell, N ;

Anyim, M ;

Prentice, MB ;

Nye, KE ;

Wren, B ;

Morrow, WJW .

VACCINE, 2004, 22 (20) :2651-2659

[9] Identification of an antigenic epitope in Helicobacter pylori urease that induces neutralizing antibody production [J].

Hirota, K ;

Nagata, K ;

Norose, Y ;

Futagami, S ;

Nakagawa, Y ;

Senpuku, H ;

Kobayashi, M ;

Takahashi, H .

INFECTION AND IMMUNITY, 2001, 69 (11) :6597-6603

[10] ORAL IMMUNIZATION WITH RECOMBINANT HELICOBACTER-PYLORI UREASE INDUCES SECRETORY IGA ANTIBODIES AND PROTECTS MICE FROM CHALLENGE WITH HELICOBACTER-FELIS [J].

LEE, CK ;

WELTZIN, R ;

THOMAS, WD ;

KLEANTHOUS, H ;

ERMAK, TH ;

SOMAN, G ;

HILL, JE ;

ACKERMAN, SK ;

MONATH, TP .

JOURNAL OF INFECTIOUS DISEASES, 1995, 172 (01) :161-172