Microfluidic array for three-dimensional perfusion culture of human mammary epithelial cells

被引：53

作者：

Chen, Shin-Yi Cindy ^{[1
]}

Hung, Paul J. ^{[1
]}

Lee, Philip J. ^{[1
]}

机构：

[1] CellASIC Corp, Hayward, CA 94545 USA

来源：

BIOMEDICAL MICRODEVICES | 2011年 / 13卷 / 04期

关键词：

Microfluidics; 3D cell culture; Mammary epithelial cells; MCF-10A; IN-VITRO EVALUATION; GENE-EXPRESSION; MATRIX; MODEL; DRUGS; ACINI;

D O I：

10.1007/s10544-011-9545-3

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

The ability to culture cells in three dimensional extracellular matrix (3D ECM) has proven to be an important tool for laboratory biology. Here, we demonstrate a microfluidic perfusion array on a 96-well plate format capable of long term 3D ECM culture within biomimetic microchambers. The array consists of 32 independent flow units, each with a 4 mu l open-top culture chamber, and 350 mu l inlet and outlet wells. Perfusion is generated using gravity and surface tension forces, allowing the array to be operated without any external pumps. MCF-10A mammary epithelial cells cultured in Matrigel in the microfluidic array exhibit acinus morphology over 9 days consistent with previous literature. We further demonstrated the application of the microfluidic array for in vitro anti-cancer drug screening.

引用

页码：753 / 758

页数：6

共 18 条

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