Regulation of the level of vesl-1S/homer-1a proteins by ubiquitin-proteasome proteolytic systems

被引:38
作者
Ageta, H
Kato, A
Hatakeyama, S
Nakayama, K
Isojima, Y
Sugiyama, H [1 ]
机构
[1] Kyushu Univ, Grad Sch Sci, Dept Biol, Higashi Ku, Fukuoka 8128581, Japan
[2] Kyushu Univ, Grad Sch Med Sci, Dept Mol Biol, Higashi Ku, Fukuoka 8128582, Japan
[3] Kyushu Univ, Med Inst Bioregulat, Lab Embryon & Genet Engn, Dept Mol & Cellular Biol,Higashi Ku, Fukuoka 8128582, Japan
[4] Osaka Univ, Inst Prot Res, Div Prot Metab, Suita, Osaka 5650871, Japan
关键词
D O I
10.1074/jbc.M011097200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The vesl-1S/homer-1a gene is up-regulated during seizure and long term potentiation. Other members of the Vesl family, Vesl-1L, -2, and -3, are constitutively expressed in the brain. We examined the regulatory mechanisms governing the expression level of Vesl-1S protein, either an exogenously introduced one in COS7 or human embryonic kidney 293T cells or an endogenous one in rat brain neurons in cultures, In both cases, application of proteasome inhibitors increased the amount of Vesl-1S protein but not that of Vesl-1L, -2, or -3 protein. Deletion analyses revealed that the C-terminal 11-amino acid region was responsible for the proteolysis of Vesl-1S by proteasomes. Application of proteasome inhibitors promoted ubiquitination of Vesl-1S protein but not that of the Vesl-1S deletion mutant, which evaded proteasome-mediated degradation. These results indicate that ubiquitin-proteasome systems are involved in the regulation of the expression level of Vesl-1S protein.
引用
收藏
页码:15893 / 15897
页数:5
相关论文
共 36 条
[21]   A role for the proteasome in the light response of the timeless clock protein [J].
Naidoo, N ;
Song, W ;
Hunter-Ensor, M ;
Sehgal, A .
SCIENCE, 1999, 285 (5434) :1737-1741
[22]   Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin [J].
Naisbitt, S ;
Kim, E ;
Tu, JC ;
Xiao, B ;
Sala, C ;
Valtschanoff, J ;
Weinberg, RJ ;
Worley, PF ;
Sheng, M .
NEURON, 1999, 23 (03) :569-582
[23]   REQUIREMENT OF A CRITICAL PERIOD OF TRANSCRIPTION FOR INDUCTION OF A LATE-PHASE OF LTP [J].
NGUYEN, PV ;
ABEL, T ;
KANDEL, ER .
SCIENCE, 1994, 265 (5175) :1104-1107
[24]   THE 2ND-CODON RULE AND AUTOPHOSPHORYLATION GOVERN THE STABILITY AND ACTIVITY OF MOS DURING THE MEIOTIC CELL-CYCLE IN XENOPUS-OOCYTES [J].
NISHIZAWA, M ;
OKAZAKI, K ;
FURUNO, N ;
WATANABE, N ;
SAGATA, N .
EMBO JOURNAL, 1992, 11 (07) :2433-2446
[25]   Cell cycle regulation by the ubiquitin pathway [J].
Pagano, M .
FASEB JOURNAL, 1997, 11 (13) :1067-1075
[26]  
Rechsteiner M, 1996, TRENDS BIOCHEM SCI, V21, P267, DOI 10.1016/0968-0004(96)10031-1
[27]   Homer 1b regulates the trafficking of group I metabotropic glutamate receptors [J].
Roche, KW ;
Tu, JC ;
Petralia, RS ;
Xiao, B ;
Wenthold, RJ ;
Worley, PF .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (36) :25953-25957
[28]   New insights into the regulation of protein kinase C and novel phorbol ester receptors [J].
Ron, D ;
Kazanietz, MG .
FASEB JOURNAL, 1999, 13 (13) :1658-1676
[29]   Common pathway for the ubiquitination of IκBα, IκBβ, and IκBε mediated by the F-box protein FWD1 [J].
Shirane, M ;
Hatakeyama, S ;
Hattori, K ;
Nakayama, K ;
Nakayama, K .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (40) :28169-28174
[30]   MEMORY IMPAIRMENT DURING PROLONGED TRAINING IN MICE GIVEN INHIBITORS OF CEREBRAL PROTEIN-SYNTHESIS [J].
SQUIRE, LR ;
BARONDES, SH .
BRAIN RESEARCH, 1973, 56 (JUN29) :215-225