fixation;
fluorescence in situ hybridization;
human embryos;
preimplantation genetic diagnosis;
D O I:
10.1023/A:1022542406056
中图分类号:
Q3 [遗传学];
学科分类号:
071007 ;
090102 ;
摘要:
Purpose: Our purpose was to modify, a fixation method using Tween-20 and HCl (TH) and to compare it with a protocol using methanol and acetic acid (MA) for the improvement of preimplantation genetic diagnosis by fluorescence in situ hybridization (FISH). Methods: Single blastomeres were allocated to either the TH or the MA procedure. The two methods were compared to evaluate efficiency of fixation and the intensities of FISH signals, Results: With the TH method, 123 (93.9%) of 131 blastomeres were fixed, while only 95 (78.5%) of 121 were fixed with MA. Average scores far the intensity of FISH signals were significantly stronger for TH than for MA (P < 0.05). There was also a significant difference in signal intensity scores between the two methods for type-3 nuclei. Conclusions: Our results indicate that not only are fewer nuclei last during fixation but also stronger FISH signals can be obtained with the TH method. Thus, modified TH can improve the overall efficiency of preimplantation genetic diagnosis.
机构:The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York
MUNNE, S
;
WEIER, HUG
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机构:The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York
WEIER, HUG
;
STEIN, J
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机构:The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York
STEIN, J
;
GRIFO, J
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机构:The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York
GRIFO, J
;
COHEN, J
论文数: 0引用数: 0
h-index: 0
机构:The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York
机构:The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York
MUNNE, S
;
WEIER, HUG
论文数: 0引用数: 0
h-index: 0
机构:The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York
WEIER, HUG
;
STEIN, J
论文数: 0引用数: 0
h-index: 0
机构:The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York
STEIN, J
;
GRIFO, J
论文数: 0引用数: 0
h-index: 0
机构:The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York
GRIFO, J
;
COHEN, J
论文数: 0引用数: 0
h-index: 0
机构:The Gamete and Embryo Research Laboratory, The Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York