Serology of Orthopoxvirus cameli infection in dromedary camels: Analysis by ELISA and western blotting

被引:20
作者
Azwai, SM
Carter, SD
Woldehiwet, Z
Wernery, U
机构
[1] UNIV LIVERPOOL, DEPT VET PATHOL, LIVERPOOL L69 3BX, MERSEYSIDE, ENGLAND
[2] UNIV LIVERPOOL, DEPT VET CLIN SCI & ANIM HUSB, LIVERPOOL L69 3BX, MERSEYSIDE, ENGLAND
[3] CENT VET RES LAB, DUBAI, U ARAB EMIRATES
关键词
camel; dromedary; Camelus dromedarius; Orthopoxvirus cameli; camelpox; contagious ecthyma; ELISA; monoclonal antibodies; Western blotting; Libya;
D O I
10.1016/0147-9571(95)00023-2
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
An enzyme-linked immunosorbent assay (ELISA) was developed, together with a Western blotting technique, for the detection of total and IgG and IgM antibodies to camelpox virus (Orthopoxvirus cameli) in camel (Camelus dromedarius) sera and for identifying the seroreactive antigens of the virus. A total of 520 camels from different regions in Libya were tested. The overall seropositivity rate in the examined herds was 9.8%, and varied between herds from 0 to 30%. Two viral antigenic determinants (31 and 35 kDa) were shared by the Western blotting patterns of all the positive camel sera tested. The developed ELISA assay showed ability to differentiate between orthopox and parapoxvirus infections in camels. It is considered that the ELISA technique is justified for serodiagnosis of camelpox in the camel and could be easily modified and usefully applied to other species at risk of poxvirus infection.
引用
收藏
页码:65 / 78
页数:14
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