THE ISOLATION AND CHARACTERIZATION OF CAMEL (CAMELUS-DROMEDARIUS) IMMUNOGLOBULIN CLASSES AND SUBCLASSES

被引：25

作者：

AZWAI, SM ^{[1
]}

CARTER, SD ^{[1
]}

WOLDEHIWET, Z ^{[1
]}

机构：

[1] UNIV LIVERPOOL,DEPT VET CLIN SCI & ANIM HUSB,LIVERPOOL L69 3BX,ENGLAND

来源：

JOURNAL OF COMPARATIVE PATHOLOGY | 1993年 / 109卷 / 02期

关键词：

D O I：

10.1016/S0021-9975(08)80262-9

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Immunoglobulins G and M were prepared from camel sera by ammonium sulphate precipitation, gel filtration and fast protein liquid chromatography (ion exchange). Heavy and light chains were identified by polyacrylamide gel electrophoresis and Western blotting. IgG subclasses were isolated by DEAE ion exchange chromatography and shown to have different electrophoretic motilities. No molecules corresponding to IgA were detected. Rabbit anti-serum against camel IgG was generated and its specificity shown by Western blotting. © 1993, Academic Press Limited. All rights reserved.

引用

页码：187 / 195

页数：9

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