NSF is required for the brefeldin A-promoted disassembly of the Golgi apparatus

被引：0

作者：

Fukunaga, T

Furuno, A

Hatsuzawa, K

Tani, K

Yamamoto, A

Tagaya, M ^{[1
]}

机构：

[1] Tokyo Univ Pharm & Life Sci, Sch Life Sci, 1432-1 Horinouchi, Hachioji, Tokyo 1920392, Japan

[2] Kansai Med Univ, Dept Physiol, Moriguchi, Osaka 5700074, Japan

来源：

FEBS LETTERS | 1998年 / 435卷 / 2-3期

关键词：

brefeldin A; Golgi apparatus; microinjection; retrograde transport; N-ethylmaleimide-sensitive factor;

D O I：

10.3390/cells11060938

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

N-Ethylmaleimide-sensitive factor (NSF) is required for multiple pathways of vesicle-mediated protein transport. Microinjection of a monoclonal anti-NSF antibody almost completely blocked brefeldin A-promoted Golgi disassembly without affecting the rapid release of beta-COP, a subunit of the Golgi coat proteins (COPI), from the Golgi apparatus. Similar results mere obtained using a dominant-negative NSF which is known to compete with endogenous NSF, The present results suggest that an NSF-mediated step is present in the brefeldin A-promoted disassembly of the Golgi apparatus. (C) 1998 Federation of European Biochemical Societies.

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页码：237 / 240

页数：4

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