Estimating genetic diversity in durum and bread wheat cultivars from Turkey using AFLP and SAMPL markers

被引：59

作者：

Altintas, S. ^{[1
]}

Toklu, F. ^{[2
]}

Kafkas, S. ^{[3
]}

Kilian, B. ^{[4
]}

Brandolini, A. ^{[5
]}

Ozkan, H. ^{[1
]}

机构：

[1] Cukurova Univ, Fac Agr, Dept Field Crops, TR-01330 Adana, Turkey

[2] Univ Cukurova Kozan, Vocat Sch Kozan, Seed Sci & Technol Program, Adana, Turkey

[3] Cukurova Univ, Fac Agr, Dept Hort, TR-01330 Adana, Turkey

[4] Max Planck Inst Plant Breeding Res, Cologne, Germany

[5] Ist Sperimentale Cerealicoltura, CRA, I-26886 San Angelo Lodigiano, Italy

来源：

PLANT BREEDING | 2008年 / 127卷 / 01期

关键词：

Turkey; durum wheat; bread wheat; AFLP; SAMPL;

D O I：

10.1111/j.1439-0523.2007.01424.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Since 1925, more than 100 wheat varieties were developed and released in Turkey, and many more were introduced from abroad, but no systematic analysis of their genetic diversity has been performed yet. In this research, a total of 34 domestic and foreign cultivars (12 durum and 22 bread wheats), released in Turkey between 1936 and 2000, were fingerprinted by means of five amplified fragment length polymorphism and three selective amplification of microsatellite polymorphic loci (SAMPL) primer combinations, to evaluate their genetic variation and to determine the existence of cultivar-specific bands. Among the 344 amplicons scored, 214 were polymorphic. The primer combination E-ACG/M-AGG yielded the highest number and the primer combination SAMPL-6/M-AGA produced the lowest number of polymorphic bands. Most cultivars were molecularly very similar, although a few distinct ones (the durum wheat 'Kunduru-1149' and the bread wheat 'Ikizce-96') were also identified. Seven cultivar-specific markers for different bread wheat cultivars ('Golia', 'Seri-82', 'Adana-99', 'Pandas' and 'Sertak-52') and six cultivar-specific markers for durum wheat cv 'Kunduru' were observed. Our results show that genetic diversity among old and present-day wheat cultivar commonly grown in Turkey is limited.

引用

页码：9 / 14

页数：6

共 40 条

[21] Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers [J].

Nagaoka, T ;

Ogihara, Y .

THEORETICAL AND APPLIED GENETICS, 1997, 94 (05) :597-602

[22] MATHEMATICAL-MODEL FOR STUDYING GENETIC-VARIATION IN TERMS OF RESTRICTION ENDONUCLEASES [J].

NEI, M ;

LI, WH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (10) :5269-5273

[23] A reconsideration of the domestication geography of tetraploid wheats [J].

Ozkan, H ;

Brandolini, A ;

Pozzi, C ;

Effgen, S ;

Wunder, J ;

Salamini, F .

THEORETICAL AND APPLIED GENETICS, 2005, 110 (06) :1052-1060

[24] Morphological and molecular characterization of Italian emmer wheat accessions [J].

Pagnotta, MA ;

Mondini, L ;

Atallah, M .

EUPHYTICA, 2005, 146 (1-2) :29-37

[25] Genetic diversity in Australian wheat varieties and breeding material based on RFLP data [J].

Paull, JG ;

Chalmers, KJ ;

Karakousis, A ;

Kretschmer, JM ;

Manning, S ;

Langridge, P .

THEORETICAL AND APPLIED GENETICS, 1998, 96 (3-4) :435-446

[26] DETECTION OF GENETIC DIVERSITY IN CLOSELY-RELATED BREAD WHEAT USING MICROSATELLITE MARKERS [J].

PLASCHKE, J ;

GANAL, MW ;

RODER, MS .

THEORETICAL AND APPLIED GENETICS, 1995, 91 (6-7) :1001-1007

[27] The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis [J].

Powell, W ;

Morgante, M ;

Andre, C ;

Hanafey, M ;

Vogel, J ;

Tingey, S ;

Rafalski, A .

MOLECULAR BREEDING, 1996, 2 (03) :225-238

[28] A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars [J].

Prevost, A ;

Wilkinson, MJ .

THEORETICAL AND APPLIED GENETICS, 1999, 98 (01) :107-112

[29]

Rakoczy-Trojanowska M, 2004, CELL MOL BIOL LETT, V9, P221

[30]

Rholf FJ, 1993, NTSYS PC NUMERICAL T

← 1 2 3 4 →