Time-resolved fluorometric assay for measuring cell binding and association of native and oxidized low-density lipoproteins to macrophages

被引：13

作者：

Wang, XS ^{[1
]}

Greilberger, J ^{[1
]}

Jürgens, G ^{[1
]}

机构：

[1] Karl Franzens Univ Graz, Inst Med Biochem, A-8010 Graz, Austria

来源：

ANALYTICAL BIOCHEMISTRY | 1999年 / 267卷 / 02期

基金：

奥地利科学基金会;

关键词：

D O I：

10.1006/abio.1998.3023

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Europium-labeled native and oxidized low-density lipoprotein (LDL) were used to measure their binding and cell association to mouse peritoneal macrophages, to suspended human monocyte cell line THP-1 cells, and to differentiated THP-1 macrophages. Cell binding and association were concentration dependent and saturable and showed the characteristics of Ligand-receptor interaction The validity of this assay was also supported by comparison with the method using (125)iodine-labeled LDL. This nonradioactive assay proved to be specific, sensitive and simple and avoided any potential lipid peroxidation of LDL brought about by labeling lipoproteins with the widely used radioactive iodine. The latter fact is very important in studying lipoprotein-receptor interactions. (C) 1999 Academic Press.

引用

页码：271 / 278

页数：8

共 41 条

[11]

GERRITY RG, 1981, AM J PATHOL, V103, P181

[12]

GOLDSTEIN JL, 1983, METHOD ENZYMOL, V98, P241

[13] BINDING-SITE ON MACROPHAGES THAT MEDIATES UPTAKE AND DEGRADATION OF ACETYLATED LOW-DENSITY LIPOPROTEIN, PRODUCING MASSIVE CHOLESTEROL DEPOSITION [J].

GOLDSTEIN, JL ;

HO, YK ;

BASU, SK ;

BROWN, MS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (01) :333-337

[14] In vitro interactions of oxidatively modified LDL with type I, II, III, IV and V collagen, laminin, fibronectin, and poly-D-lysine [J].

Greilberger, J ;

Schmut, O ;

Jurgens, G .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1997, 17 (11) :2721-2728

[15] ENHANCED MACROPHAGE DEGRADATION OF LOW-DENSITY LIPOPROTEIN PREVIOUSLY INCUBATED WITH CULTURED ENDOTHELIAL-CELLS - RECOGNITION BY RECEPTORS FOR ACETYLATED LOW-DENSITY LIPOPROTEINS [J].

HENRIKSEN, T ;

MAHONEY, EM ;

STEINBERG, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1981, 78 (10) :6499-6503

[16]

Innerarity T L, 1986, Methods Enzymol, V129, P542

[17]

JURGENS G, 1992, CLIN CHEM, V38, P853

[18]

Karp M T, 1983, J Appl Biochem, V5, P399

[19]

KHOUW AS, 1993, J LIPID RES, V34, P1483

[20] STRUCTURES AND FUNCTIONS OF MULTILIGAND LIPOPROTEIN RECEPTORS - MACROPHAGE SCAVENGER RECEPTORS AND LDL RECEPTOR-RELATED PROTEIN (LRP) [J].

KRIEGER, M ;

HERZ, J .

ANNUAL REVIEW OF BIOCHEMISTRY, 1994, 63 :601-637

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