A 1-Mb BAC/PAC-Based physical map of the autosomal recessive polycystic kidney disease gene (PKHD1) region on chromosome 6

被引：14

作者：

Park, JH

Dixit, MP

Onuchic, LF

Wu, GQ

Goncharuk, AN

Kneitz, S

Santarina, LB

Hayashi, T

Avner, ED

Guay-Woodford, L

Zerres, K

Germino, GG

Somlo, S

机构：

[1] Albert Einstein Coll Med, Dept Med, Bronx, NY 10461 USA

[2] Albert Einstein Coll Med, Dept Mol Genet, Bronx, NY 10461 USA

[3] Johns Hopkins Univ, Sch Med, Dept Med, Baltimore, MD 21205 USA

[4] Case Western Reserve Univ, Rainbow Babies & Childrens Hosp, Dept Pediat, Cleveland, OH 44106 USA

[5] Univ Alabama, Dept Pediat, Birmingham, AL 35294 USA

[6] Univ Alabama, Dept Med, Birmingham, AL 35294 USA

[7] Univ Bonn, Inst Human Genet, D-5111 Bonn, Germany

来源：

GENOMICS | 1999年 / 57卷 / 02期

关键词：

D O I：

10.1006/geno.1999.5777

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The PKHD1 (polycystic kidney and hepatic disease 1) gene responsible for autosomal recessive polycystic kidney disease has been mapped to 6p21.1-p12 to an similar to 1-cM interval flanked by the markers D6S1714/D6S243 and D6S1024. We have developed a sequence-ready BAG/PAC-based contig map of this region as the next step for the positional cloning of PKHD1. This contig comprising 52 clones spanning similar to 1 Mb was established by content mapping of 44 BAG/PAC-end-derived STSs, 3 known genetic markers, 5 YAC-end-derived STSs, 3 random STSs, 1 previously mapped gene, and 1 EST. The average depth per marker is 6.3 clones, and the average STS density is 20 kb. The genomic clone overlaps were confirmed by restriction fragment fingerprint analysis. A high-resolution BAC/PAC-based contig map is essential to the ultimate goal of identifying the PKHD1 gene. (C) 1999 Academic Press.

引用

页码：249 / 255

页数：7

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