Cyanine dye conjugates as probes for live cell imaging

被引：75

作者：

Carreon, Jay R.

Stewart, Kelly M.

Mahon, Kerry P., Jr.

Shin, Stephanie

Kelley, Shana O. ^{[1
]}

机构：

[1] Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada

[2] Boston Coll, Eugene F Merkert Chem Ctr, Chestnut Hill, MA 02467 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2007年 / 17卷 / 18期

基金：

美国国家科学基金会;

关键词：

peptide conjugate; fluorescence imaging; cellular imaging; dyes;

D O I：

10.1016/j.bmcl.2007.06.097

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

A series of fluorescent compounds suitable for live cell imaging is described. Functionalized forms of four different asymmetric cyanine dyes are reported that are amenable to peptide conjugation. The photophysical properties of the modified dyes and conjugates and the use of the compounds as cellular imaging agents are described. The results obtained indicate that these spectrally versatile compounds, which have absorption and emission profiles spanning the visible spectrum, are useful probes for cellular imaging. (c) 2007 Elsevier Ltd. All rights reserved.

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页码：5182 / 5185

页数：4

相关论文

共 13 条

[1]

[Anonymous], [No title captured]

[2] HETERODIMERIC DNA-BINDING DYES DESIGNED FOR ENERGY-TRANSFER - SYNTHESIS AND SPECTROSCOPIC PROPERTIES [J].

BENSON, SC ;

SINGH, P ;

GLAZER, AN .

NUCLEIC ACIDS RESEARCH, 1993, 21 (24) :5727-5735

[3] Cyanine dyes with high absorption cross section as donor chromophores in energy transfer primers [J].

Hung, SC ;

Ju, JY ;

Mathies, RA ;

Glazer, AN .

ANALYTICAL BIOCHEMISTRY, 1996, 243 (01) :15-27

[4] Solid-phase synthesis of asymmetric cyanine dyes [J].

Isacsson, J ;

Westman, G .

TETRAHEDRON LETTERS, 2001, 42 (18) :3207-3210

[5] Positively charged DNA-binding proteins cause apparent cell membrane translocation [J].

Lundberg, M ;

Johansson, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2002, 291 (02) :367-371

[6] Cyanines during the 1990s: A review [J].

Mishra, A ;

Behera, RK ;

Behera, PK ;

Mishra, BK ;

Behera, GB .

CHEMICAL REVIEWS, 2000, 100 (06) :1973-2011

[7] Base-content dependence of emission enhancements, quantum yields, and lifetimes for cyanine dyes bound to double-strand DNA: Photophysical properties of monomeric and bichromophoric DNA stains [J].

Netzel, TL ;

Nafisi, K ;

Zhao, M ;

Lenhard, JR ;

Johnson, I .

JOURNAL OF PHYSICAL CHEMISTRY, 1995, 99 (51) :17936-17947

[8] Synthesis and binding properties of oligo-2′-deoxyribonucleotides covalently linked to a thiazole orange derivative [J].

Privat, E ;

Asseline, U .

BIOCONJUGATE CHEMISTRY, 2001, 12 (05) :757-769

[9] Cell-penetrating peptides - A reevaluation of the mechanism of cellular uptake [J].

Richard, JP ;

Melikov, K ;

Vives, E ;

Ramos, C ;

Verbeure, B ;

Gait, MJ ;

Chernomordik, LV ;

Lebleu, B .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (01) :585-590

[10] Experimental and computational investigation of unsymmetrical cyanine dyes: Understanding torsionally responsive fluorogenic dyes [J].

Silva, Gloria L. ;

Ediz, Volkan ;

Yaron, David ;

Armitage, Bruce A. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (17) :5710-5718