High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells

被引：274

作者：

Raymond, Christopher S. ^{[1
]}

Soriano, Philippe ^{[1
]}

机构：

[1] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Program Dev Biol, Seattle, WA 98104 USA

来源：

PLOS ONE | 2007年 / 2卷 / 01期

关键词：

D O I：

10.1371/journal.pone.0000162

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

DNA site-specific recombinases (SSRs) such as Cre, FLPe, and Phi C31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and Phi C31 (Phi C31o) SSRs, which result in recombination efficiencies similar to Cre.

引用

页数：4

共 16 条

[1] Enhanced efficiency through nuclear localization signal fusion on phage φC31-integrase:: activity comparison with Cre and FLPe recombinase in mammalian cells [J].

Andreas, S ;

Schwenk, F ;

Küter-Luks, B ;

Faust, N ;

Kühn, R .

NUCLEIC ACIDS RESEARCH, 2002, 30 (11) :2299-2306

[2] An Flp indicator mouse expressing alkaline phosphatase from the ROSA26 locus [J].

Awatramani, R ;

Soriano, P ;

Mai, JJ ;

Dymecki, S .

NATURE GENETICS, 2001, 29 (03) :257-259

[3] Site-specific cassette exchange and germline transmission with mouse ES cells expressing φC31 integrase [J].

Belteki, G ;

Gertsenstein, M ;

Ow, DW ;

Nagy, A .

NATURE BIOTECHNOLOGY, 2003, 21 (03) :321-324

[4] Talking about a revolution: The impact of site-specific recombinases on genetic analyses in mice [J].

Branda, CS ;

Dymecki, SM .

DEVELOPMENTAL CELL, 2004, 6 (01) :7-28

[5] Different thermostabilities of FLP and Cre recombinases: Implications for applied site-specific recombination [J].

Buchholz, F ;

Ringrose, L ;

Angrand, PO ;

Rossi, F ;

Stewart, AF .

NUCLEIC ACIDS RESEARCH, 1996, 24 (21) :4256-4262

[6] Improved properties of FLP recombinase evolved by cycling mutagenesis [J].

Buchholz, F ;

Angrand, PO ;

Stewart, AF .

NATURE BIOTECHNOLOGY, 1998, 16 (07) :657-662

[7] Flp recombinase promotes site-specific DNA recombination in embryonic stem cells and transgenic mice [J].

Dymecki, SM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) :6191-6196

[8] A phage integrase directs efficient site-specific integration in human cells [J].

Groth, AC ;

Olivares, EC ;

Thyagarajan, B ;

Calos, MP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (11) :5995-6000

[9] Codon bias and heterologous protein expression [J].

Gustafsson, C ;

Govindarajan, S ;

Minshull, J .

TRENDS IN BIOTECHNOLOGY, 2004, 22 (07) :346-353

[10] φc31 integrase induces chromosomal aberrations in primary human fibroblasts [J].

Liu, J. ;

Jeppesen, I. ;

Nielsen, K. ;

Jensen, T. G. .

GENE THERAPY, 2006, 13 (15) :1188-1190

← 1 2 →