Enhancement of MSC adhesion and therapeutic efficiency in ischemic heart using lentivirus delivery with periostin

被引：44

作者：

Cho, Yun-Hyeong ^{[2
,3
]}

Cha, Min-Ji ^{[3
,4
]}

Song, Byeong-Wook ^{[3
,4
]}

Kim, Il-Kwon ^{[3
,4
]}

Song, Heesang ^{[5
]}

Chang, Woochul ^{[6
]}

Lim, Soyeon ^{[7
]}

Ham, Onju ^{[3
,4
]}

Lee, Se-Yeon ^{[3
,4
]}

Choi, Eunmi ^{[1
]}

Kwon, Hyuck Moon ^{[4
,8
]}

Hwang, Ki-Chul ^{[1
,3
,4
]}

机构：

[1] Yonsei Univ, Coll Med, Severance Biomed Sci Inst, Seoul 120752, South Korea

[2] Kwandong Univ, Coll Med, Myongji Hosp, Div Cardiol, Goyangsi 412270, Gyeonggido, South Korea

[3] Yonsei Univ, Coll Med, Cardiovasc Res Inst, Seoul 120752, South Korea

[4] Yonsei Univ, Coll Med, Brain Korea Project Med Sci 21, Seoul 120752, South Korea

[5] Chosun Univ, Sch Med, Dept Biochem & Mol Biol, Kwangju 501717, South Korea

[6] Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06520 USA

[7] Univ Rochester, Sch Med & Dent, Cardiovasc Res Inst, Rochester, NY 14642 USA

[8] Yonsei Univ, Coll Med, Gangnam Severance Hosp, Seoul 135720, South Korea

来源：

BIOMATERIALS | 2012年 / 33卷 / 05期

关键词：

Adhesion; Infarcted myocardium; Lentivirus; Mesenchymal stem cells; Periostin; MESENCHYMAL STEM-CELLS; NEONATAL-RAT CARDIOMYOCYTES; MYOCARDIAL-INFARCTION; CALCIUM HOMEOSTASIS; SIGNAL-TRANSDUCTION; PROGENITOR CELLS; CANCER CELLS; LUNG-CANCER; HYPOXIA; EXPRESSION;

D O I：

10.1016/j.biomaterials.2011.10.078

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

Many approaches have shown beneficial effects of modified mesenchymal stem cells (MSCs) for treatment of infarcted myocardium, but have primarily focused on enhancing the survival of transplanted MSCs. Here, we show the dual benefits of periostin-overexpressing MSCs (p-MSCs) for infarcted myocardium. P-MSCs led to the marked histological and functional recovery of infarcted myocardium by enhancing survival of MSCs and directly preventing apoptosis of cardiomyocytes. Survival of p-MSCs themselves and cardiomyocytes co-cultured with p-MSCs or treated with the conditioned media from p-MSCs was significantly increased under hypoxic conditions. Decreases in adhesion-related integrins were reversed in cardiomyocytes co-cultured with p-MSCs, followed by increases in p-PI3K and Akt, indicating that periostin activates the PI3K pathway through adhesion-related integrins. When p-MSCs were injected into myocardial infarcted rats, histological pathology and cardiac function were significantly improved compared to MSC-injected controls. Thus, periostin might be a new target of therapeutic treatments using MSCs as carriers for infarcted myocardium. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1376 / 1385

页数：10

共 36 条

[1]

Periostin promotes invasiveness and resistance of pancreatic cancer cells to hypoxia-induced cell death:: role of the β4 integrin and the PI3k pathway [J].