Microwave-assisted solid phase peptide synthesis on high loaded resins

被引：34

作者：

Coantic, Stephanie ^{[3
]}

Subra, Gilles ^{[1
,2
]}

Martinez, Jean ^{[1
,2
]}

机构：

[1] Univ Montpellier 1, CNRS, UMR 5247, Inst Biomol Max Mousseron,Fac Pharm Montpellier, F-34000 Montpellier, France

[2] Univ Montpellier 2, CNRS, UMR 5247, Inst Biomol Max Mousseron,Fac Pharm Montpellier, F-34000 Montpellier, France

[3] CEA Valrho DRCP SCPS LCAM, CNRS, UMR 5257, Inst Chim Seperat Marcoule, F-30207 Bagnols Sur Ceze, France

来源：

INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS | 2008年 / 14卷 / 02期

关键词：

SPPS; microwave-assisted peptide synthesis; resin beads; mechanical stirring;

D O I：

10.1007/s10989-008-9123-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Kinetic of diffusion of reactants from the solution into the resin beads is a key factor of the efficiency of reaction in solid phase synthesis. We demonstrated in this paper that stirring is required to obtain homogeneous solution during the short coupling steps in microwave-assisted SPPS. Different types of resin, loading rate and coupling reactants were investigated to highlight this observation.

引用

页码：143 / 147

页数：5