Molecular cloning and sequencing of a cDNA encoding dihydropyrimidinase from the rat liver

被引：22

作者：

Matsuda, K

Sakata, S

Kaneko, M

Hamajima, N

Nonaka, M

Sasaki, M

Tamaki, N

机构：

[1] KOBE GAKUIN UNIV,FAC NUTR,KOBE,HYOGO,JAPAN

[2] NAGOYA CITY UNIV,SCH MED,DEPT PEDIAT,MIZUHO KU,NAGOYA,AICHI 467,JAPAN

[3] NAGOYA CITY UNIV,SCH MED,DEPT BIOCHEM,MIZUHO KU,NAGOYA,AICHI 467,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1307卷 / 02期

关键词：

dihydropyrimidinase; cloning; sequencing; rat;

D O I：

10.1016/0167-4781(96)00056-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA encoding dihydropyrimidinase has been isolated from a rat cDNA library. The N-terminal and an internal amino acid sequences were determined, and PCR primers were designed based on these sequences. Using a cDNA fragment amplified by RT-PCR with these primers, three cDNA clones were isolated from a rat liver library. The clone with the longest insert of 2129 bp contained a 1557 bp open reading frame encoding a polypeptide of 519 residues with a molecular mass of 56 832 Da.

引用

页码：140 / 144

页数：5

共 25 条

[1] DIHYDROPYRIMIDINE AMIDOHYDROLASE IS A ZINC METALLOENZYME [J].