Ionizing radiation and hydrogen peroxide induced oxidative DNA base damage in two L5178Y cell lines

被引：13

作者：

Zastawny, TH

Kruszewski, M

Olinski, R

机构：

[1] Univ Sch Med Sci, Dept Clin Biochem, PL-85092 Bydgoszcz, Poland

[2] Inst Nucl Chem & Technol, Dept Radiobiol & Hlth Protect, PL-03195 Warsaw, Poland

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1998年 / 24卷 / 7-8期

关键词：

free radical; DNA damage; oxidative base damage; radiation sensitivity; H2O2; sensitivity;

D O I：

10.1016/S0891-5849(97)00452-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Seven oxidized DNA bases were quantified, by gas GC/MS-SIM, in chromatin from gamma-rays and H2O2 treated mouse lymphoma L5178Y (LY) cells, inversely cross-sensitive to these agents. in H2O2 treated cells (2 mM, 1 h, 37 degrees C) we found more damage in LY-R cells than in LY-S cells. On the contrary, in gamma-rays (400 Gy) treated cells we found more damaged DNA bases in LY-S cells. The yield of damaged bases in control cells was similar in both cell Lines, with the exception of 80HAde and FapyGua that were found at a much higher level in LY-S cells. The yields of damaged bases were related to cellular sensitivity to damaging agent; this observation points to a relationship between DNA base damage induction, antioxidant defense system in the intracellular milieu and cell sensitivity. (C) 1998 Elsevier Science Inc.

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页码：1250 / 1255

页数：6

相关论文

共 36 条

[11] DEFICIENCY IN DNA-REPAIR IN MOUSE LYMPHOMA STRAIN L5178Y-S [J].

EVANS, HH ;

RICANATI, M ;

HORNG, MF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7562-7566

[12] HYDROXYL RADICAL INDUCED CROSS-LINKING OF CYTOSINE AND TYROSINE IN NUCLEOHISTONE [J].

GAJEWSKI, E ;

DIZDAROGLU, M .

BIOCHEMISTRY, 1990, 29 (04) :977-980

[13] CELL CYCLE-DEPENDENT REPAIR OF DOUBLE-STRAND DNA BREAKS IN A GAMMA-RAY-SENSITIVE CHINESE-HAMSTER CELL [J].

GIACCIA, A ;

WEINSTEIN, R ;

HU, J ;

STAMATO, TD .

SOMATIC CELL AND MOLECULAR GENETICS, 1985, 11 (05) :485-491

[14]

Gutteridge J. M. C., 1989, FREE RADICALS BIOL M

[15] RADIOSENSITIVE XRS-5 AND PARENTAL CHO CELLS SHOW IDENTICAL DNA NEUTRAL FILTER ELUTION DOSE-RESPONSE - IMPLICATIONS FOR A RELATIONSHIP BETWEEN CELL RADIOSENSITIVITY AND INDUCTION OF DNA DOUBLE-STRAND BREAKS [J].

ILIAKIS, G ;

OKAYASU, R ;

SEANER, R .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1988, 54 (01) :55-62

[16] DNA SUPERCOILING CHANGES IN NUCLEOIDS FROM IRRADIATED L5178Y-S AND L5178Y-R CELLS [J].

KAPISZEWSKA, M ;

WRIGHT, WD ;

LANGE, CS ;

ROTI, JLR .

RADIATION RESEARCH, 1989, 119 (03) :569-575

[17] INDUCTION AND REJOINING OF DNA DOUBLE-STRAND BREAKS IN HUMAN CERVIX CARCINOMA CELL-LINES OF DIFFERING RADIOSENSITIVITY [J].

KELLAND, LR ;

EDWARDS, SM ;

STEEL, GG .

RADIATION RESEARCH, 1988, 116 (03) :526-538

[18] X-RAY SENSITIVE MUTANTS OF CHINESE-HAMSTER OVARY CELLS DEFECTIVE IN DOUBLE-STRAND BREAK REJOINING [J].

KEMP, LM ;

SEDGWICK, SG ;

JEGGO, PA .

MUTATION RESEARCH, 1984, 132 (5-6) :189-196

[19] COMPARISON OF EFFECTS OF IRON AND CALCIUM CHELATORS ON THE RESPONSE OF L5178Y SUBLINES TO X-RAYS AND H2O2 [J].

KRUSZEWSKI, M ;

GREEN, MHL ;

LOWE, JE ;

SZUMIEL, I .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1995, 326 (02) :155-163

[20] DNA STRAND BREAKAGE, CYTOTOXICITY AND MUTAGENICITY OF HYDROGEN-PEROXIDE TREATMENT AT 4-DEGREES-C AND 37-DEGREES-C IN L5178Y SUBLINES [J].

KRUSZEWSKI, M ;

GREEN, MHL ;

LOWE, JE ;

SZUMIEL, I .

MUTATION RESEARCH, 1994, 308 (02) :233-241

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