Microglial activation and tyrosine hydroxylase immunoreactivity in the substantia nigral region following transient focal ischemia in rats

被引:33
作者
Huh, Y
Jung, JW
Park, C
Ryu, JR
Shin, CY
Kim, WK
Ryu, JH
机构
[1] Kyung Hee Univ, Coll Pharm, Dept Oriental Pharmaceut Sci, Seoul 130701, South Korea
[2] Kyung Hee Univ, Coll Pharm, Coll Med, Dept Anat, Seoul 130701, South Korea
[3] Seoul Natl Univ, Coll Pharm, Dept Pharmacol, Seoul, South Korea
[4] Ewha Womans Univ, Coll Med, Dept Pharmacol, Seoul, South Korea
基金
新加坡国家研究基金会;
关键词
ischemia; substantia nigra; tyrosine hydroxylase; microglia; remote area;
D O I
10.1016/S0304-3940(03)00743-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The temporal profiles of the changes of dopaminergic cells and microglial activation induced by transient cerebral ischemia were investigated in the substantia nigra pars compacta (SNc) located outside ischemic areas of rat brain. Transient cerebral ischemia was induced by intraluminal occlusion of the right middle cerebral artery for 2 It and reperfusion was continued for 1, 2, 3, 4, 7, 10, 14, 28, 60, and 120 days. Dopaminergic cells immunostained with tyrosine hydroxylase (TH)-antibody in the ipsilateral SNc were significantly decreased at 7 days post-ischemia compared with those in the contralateral side (P < 0.05). However, at 60 and 120 days, there were no significant differences between ipsilateral and contralateral side of the SNc. Unlike the TH immunoreactivity, activated microglial cells immunostained with OX-42 antibody were significantly increased at 2 and 3 days and then decreased gradually until 10 days post-ischemia. Activated microglial cells were increased at 2 weeks post-ischemia, and this pattern remained until 60 days. These results suggest that the transient changes of TH-imnumoreactive cells in the SNc caused by transient focal ischemia are correlated with a biphasic microglial cell activation response. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:63 / 67
页数:5
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