Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism

被引：27

作者：

Ema, T ^{[1
]}

Fujii, T ^{[1
]}

Ozaki, M ^{[1
]}

Korenaga, T ^{[1
]}

Sakai, T ^{[1
]}

机构：

[1] Okayama Univ, Fac Engn, Dept Appl Chem, Okayama 7008530, Japan

来源：

CHEMICAL COMMUNICATIONS | 2005年 / 37期

关键词：

D O I：

10.1039/b508244g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The enantioselectivity of a Burkholderia cepacia lipase toward secondary alcohols could be both increased and decreased rationally by introducing only a single mutation on the basis of the mechanism proposed previously.

引用

页码：4650 / 4651

页数：2