Evolution of the Protein Corona of Lipid Gene Vectors as a Function of Plasma Concentration

被引：95

作者：

Caracciolo, Giulio ^{[1
]}

Pozzi, Daniela ^{[1
]}

Capriotti, Anna Laura ^{[2
]}

Cavaliere, Chiara ^{[2
]}

Foglia, Patrizia ^{[2
]}

Amenitsch, Heinz ^{[3
]}

Lagana, Aldo ^{[2
]}

机构：

[1] Univ Roma La Sapienza, Dept Mol Med, I-00161 Rome, Italy

[2] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy

[3] Austrian Acad Sci, Inst Biophys & Nanosyst Res, A-8042 Graz, Austria

来源：

LANGMUIR | 2011年 / 27卷 / 24期

关键词：

CATIONIC LIPOSOMES; NANOPARTICLES; PERFORMANCE; ADSORPTION; EFFICIENCY; HYDRATION; COMPLEX; SIZE;

D O I：

10.1021/la202912f

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

The concept that the effective unit of interest in the cell-nanomaterial interaction is the particle and its corona of associated proteins is emerging. Here we investigate the compositional evolution of the protein corona of 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) cationic liposomes (CLs) and DOTAP/DNA lipoplexes over a wide range of plasma concentrations (2.5-80%). The composition of the hard corona of lipoplexes is quite stable, but that of CLs does evolve considerably. We show that the protein corona of CLs is made of both low-affinity and competitive-binding proteins whose relative abundance changes with the plasma concentration. This result may have deep biological implications for the application of lipid-based gene vectors both in vitro and in vivo.

引用

页码：15048 / 15053

页数：6

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