Maize streak virus-resistant transgenic maize: a first for Africa

被引:44
作者
Shepherd, Dionne N.
Mangwende, Tichaona
Martin, Darren P.
Bezuidenhout, Marion
Kloppers, Frederik J.
Carolissen, Charlene H.
Monjane, Aderito L.
Rybicki, Edward P.
Thomson, Jennifer A.
机构
[1] Univ Cape Town, Dept Mol & Cell Biol, ZA-7701 Rondebosch, South Africa
[2] Univ Cape Town, Dept Pharmacol, ZA-7925 Cape Town, South Africa
[3] Univ Cape Town, Inst Infect Dis & Mol Med, ZA-7925 Cape Town, South Africa
[4] PANNAR PTY Ltd, ZA-3250 Greytown, South Africa
关键词
African biotechnology; geminivirus; maize streak virus; maize streak virus-resistant maize; maize yield improvement; pathogen-derived resistance; transgenic maize;
D O I
10.1111/j.1467-7652.2007.00279.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this article, we report transgene-derived resistance in maize to the severe pathogen maize streak virus (MSV). The mutated MSV replication-associated protein gene that was used to transform maize showed stable expression to the fourth generation. Transgenic T-2 and T-3 plants displayed a significant delay in symptom development, a decrease in symptom severity and higher survival rates than non-transgenic plants after MSV challenge, as did a transgenic hybrid made by crossing T-2 Hi-II with the widely grown, commercial, highly MSV-susceptible, white maize genotype WM3. To the best of our knowledge, this is the first maize to be developed with transgenic MSV resistance and the first all-African-produced genetically modified crop plant.
引用
收藏
页码:759 / 767
页数:9
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