Evidence that Ca2+-activated K+ channels play a major role in mediating the vascular effects of iloprost and cicaprost

被引:37
作者
Clapp, LH
Turcato, S
Hall, S
Baloch, M
机构
[1] Univ London Univ Coll, Dept Med, Wolfson Inst Biomed Res, Ctr Clin Pharmacol, London WC1E 6JJ, England
[2] St Thomas Hosp, Rayne Inst, London SE1 7EH, England
基金
英国惠康基金;
关键词
smooth muscle; vascular; iloprost; cicaprost; forskolin; iberiotoxin; K+ channel; Ca2+-activated; aorta; guinea-pig;
D O I
10.1016/S0014-2999(98)00549-4
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The role of K+ channels in mediating vasorelaxation induced by two prostacyclin analogues was investigated in guinea-pig aorta. Iloprost caused substantial relaxation of tissues contracted with phenylephrine or 25 mM K+ but not 60 mM K+. In endothelial-denuded tissues, maximal relaxations to iloprost, cicaprost or isoprenaline were inhibited by similar to 40-50% with tetraethylammonium or iberiotoxin, both blockers of large conductance Ca2+-activated KS (BKCA) channels. In contrast, the response to forskolin, an activator of adenylate cyclase was marginally inhibited by tetraethylammonium. The K-ATP channel blocker, glibenclamide significantly augmented the response to iloprost but not cicaprost. These effects were largely inhibited by the EP1 receptor antagonist, 8-chlorodibenz[b,f ][1,4]oxazepine-10(11 H)-carboxylic acid 2-[1-oxo-3(4-pyridinyl)propyl]hydrazide, monohydrochloride (SC-51089) and partially by indomethacin, suggesting that iloprost relaxation is counterbalanced by activation of EP1 receptors,in part through a constrictor prostaglandin. We conclude that BKCA channels play an important role in mediating the effects of iloprost and cicaprost and raises the possibility that cyclic AMP-independent pathways might be involved. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:215 / 224
页数:10
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