Cell cycle regulated transport controlled by alterations in the nuclear pore complex

被引:124
作者
Makhnevych, T
Lusk, CP
Anderson, AM
Aitchison, JD [1 ]
Wozniak, RW
机构
[1] Univ Alberta, Dept Cell Biol, Edmonton, AB T6G 2H7, Canada
[2] Inst Syst Biol, Seattle, WA 98103 USA
基金
加拿大健康研究院;
关键词
D O I
10.1016/S0092-8674(03)00986-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Eukaryotic cells have developed mechanisms for regulating the nuclear transport of macromolecules that control various cellular events including movement through defined stages of the cell cycle. In yeast cells, where the nuclear envelope remains intact throughout the cell cycle, these transport regulatory mechanisms must also function during mitosis. We have uncovered a mechanism for regulating transport that is controlled by M phase specific molecular rearrangements in the nuclear pore complex (NPC). These changes allow a transport inhibitory nucleoporin, Nup53p, to bind the karyopherin Kap121p specifically during mitosis, slowing its movement through the NPC and inducing cargo release. Yeast strains that possess defects in the function of Kap121p or the fidelity of the inhibitory pathway are delayed in mitosis. We propose that fluctuations in Kap121p transport mediated by the NPC contribute to controlling the subcellular distribution of molecules that direct progression through mitosis.
引用
收藏
页码:813 / 823
页数:11
相关论文
共 59 条
  • [31] Karyopherins in nuclear pore biogenesis: a role for Kap121p in the assembly of Nup53p into nuclear pore complexes
    Lusk, CP
    Makhnevych, T
    Marelli, M
    Aitchison, JD
    Wozniak, RW
    [J]. JOURNAL OF CELL BIOLOGY, 2002, 159 (02) : 267 - 278
  • [32] Transport into and out of the nucleus
    Macara, IG
    [J]. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2001, 65 (04) : 570 - +
  • [33] Acetylation of the adenovirus-transforming protein E1A determines nuclear localization by disrupting association with importin-α
    Madison, DL
    Yaciuk, P
    Kwok, RPS
    Lundblad, JR
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (41) : 38755 - 38763
  • [34] Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p
    Marelli, M
    Aitchison, JD
    Wozniak, RW
    [J]. JOURNAL OF CELL BIOLOGY, 1998, 143 (07) : 1813 - 1830
  • [35] A link between the synthesis of nucleoporins and the biogenesis of the nuclear envelope
    Marelli, M
    Lusk, CP
    Chan, H
    Aitchison, JD
    Wozniak, RW
    [J]. JOURNAL OF CELL BIOLOGY, 2001, 153 (04) : 709 - 723
  • [36] Nup153 is an M9-containing mobile nucleoporin with a novel Ran-binding domain
    Nakielny, S
    Shaikh, S
    Burke, B
    Dreyfuss, G
    [J]. EMBO JOURNAL, 1999, 18 (07) : 1982 - 1995
  • [37] NEHRBASS U, 1993, EUR J CELL BIOL, V62, P1
  • [38] Niedenthal RK, 1996, YEAST, V12, P773, DOI 10.1002/(SICI)1097-0061(19960630)12:8<773::AID-YEA972>3.3.CO
  • [39] 2-C
  • [40] A gradient of affinity for the karyopherin Kap95p along the yeast nuclear pore complex
    Pyhtila, B
    Rexach, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (43) : 42699 - 42709