Genetic typing of classical swine fever viruses from Lao PDR by analysis of the 5′ non-coding region

被引：20

作者：

Blacksell, SD

Khounsy, S

Boyle, DB

Gleeson, LJ

Westbury, HA

Mackenzie, JS

机构：

[1] CSIRO Livestock Ind, Australian Anim Hlth Lab, Geelong, Vic 3220, Australia

[2] Univ Queensland, Dept Microbiol & Parasitol, Brisbane, Qld, Australia

[3] Mahidol Univ, Fac Trop Med, Wellcome Trust Mahidol Univ Oxford Trop Med Res P, Bangkok 10400, Thailand

来源：

VIRUS GENES | 2005年 / 31卷 / 03期

关键词：

Asia; classical swine fever virus; genetic typing; Lao PDR; 5 ' non-coding region; pig;

D O I：

10.1007/s11262-005-3253-0

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The 5' non-coding region (5'-NCR) of 27 classical swine fever virus (CSFV) isolates from Lao People's Democratic Republic (Lao PDR) during 1997 and 1999 were amplified by RT-PCR. A 150-bp region of the 5'-NCR amplicons was analysed and compared with reference CSFV of European and Asian origin and a phylogenetic dendrogram constructed. Following analysis, all viruses were determined to belong to genogroup 2. Viruses from Lao PDR grouped on a geographical basis with the majority of northern/central isolates falling into subgroup 2.1 and southern/central isolates falling into subgroup 2.2. These results concur with previous studies of CSF viruses from Lao PDR, although this study recognized the first occurrence of subgroup 2.1 in southern Lao PDR.

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收藏

页码：349 / 355

页数：7

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