Aggregation behavior of Candida rugosa lipase

被引：38

作者：

Liou, YC ^{[1
]}

Marangoni, AG ^{[1
]}

Yada, RY ^{[1
]}

机构：

[1] Univ Guelph, Dept Food Sci, Guelph, ON N1G 2W1, Canada

来源：

FOOD RESEARCH INTERNATIONAL | 1998年 / 31卷 / 03期

基金：

加拿大自然科学与工程研究理事会;

关键词：

lipase; aggregation; activity;

D O I：

10.1016/S0963-9969(98)00099-4

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Candida rugosa lipase aggregated in aqueous media. The emission maximum wavelengths for both intrinsic tryptophan fluorescence and surface hydrophobicity, determined using the probe 8-anilino-1-naphthalene sulfonate, were blue-shifted upon aggregation of CRL. Dynamic light scattering also showed an increase in the molecular weight of CRL as a function of increasing protein concentration. Apparent dissociation constants derived from the three techniques were 0.48, 3.5 and 1.9 mu g/ml, respectively. The specific activity of aggregated species (3135 U/mg) was higher than the monomer's (860 U/mg). The aggregation state of lipases may influence kinetic properties, specificity and interfacial behavior. (C) 1999 Canadian Institute of Food Science and Technology. Published by Elsevier Science Ltd. All rights reserved.

引用

页码：243 / 248

页数：6

共 19 条

[1] CATALYTIC ACTIVITY AND ASSOCIATION OF PANCREATIC LIPASE [J].