Simultaneous determination of chloramphenicol and ketoprofen in meat and milk and chloramphenicol in egg, honey, and urine using liquid chromatography-mass spectrometry

A liquid chromatographic-atmospheric pressure ionization ion spray method is described for the determination of chloramphenicol and ketoprofen in meat and milk, chloramphenicol in egg, honey, and urine. The samples were extracted with acetonitrile (acetone for urine), the organic layer was separated from water with chloroform and evaporated to dryness, and then purified using LMS solid phase extraction columns. The method is simple, requiring only small quantities of reagents and involves minimal manual work-up procedures.