Selective human serum albumin sensor from the screening of a fluorescent rosamine library

被引：53

作者：

Ahn, Young-Hoon ^{[1
]}

Lee, Jun-Seok ^{[1
,2
,3
]}

Chang, Young-Tae ^{[1
,2
,3
,4
]}

机构：

[1] NYU, Dept Chem, New York, NY 10003 USA

[2] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[3] Natl Univ Singapore, NUS MedChem Program, Off Life Sci, Singapore 117543, Singapore

[4] ASTAR, Singapore Bioimaging Consortium, Lab Bioimaging Probe Dev, Biopolis 138667, Singapore

来源：

JOURNAL OF COMBINATORIAL CHEMISTRY | 2008年 / 10卷 / 03期

关键词：

D O I：

10.1021/cc800017h

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A fluorescent dye library approach for the development of a bioanalyte sensor was sought. The screening of a rosamine dye library against diverse macromolecules led to the discovery of a highly sensitive human serum albumin binder, G13, with similar to 36-fold fluorescence intensity change. G13 showed a highly selective response to HSA over other macromolecules including albumins from other species. The potential use of G13 for the detection of HSA in biofluids is described.

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收藏

页码：376 / 380

页数：5

相关论文

共 27 条

[1] Combinatorial rosamine library and application to in vivo glutathione probe [J].

Ahn, Young-Hoon ;

Lee, Jun-Seok ;

Chang, Young-Tae .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (15) :4510-+

[2] Solvent effect on absolute fluorescence quantum yield of rhodamine 6G determined using transient thermal lens technique [J].

Bindhu, CV ;

Harilal, SS ;

Nampoori, VPN ;

Vallabhan, CPG .

MODERN PHYSICS LETTERS B, 1999, 13 (16) :563-576

[3] Fluorescent sensors for Zn2+ based on a fluorescein platform:: Synthesis, properties and intracellular distribution [J].

Burdette, SC ;

Walkup, GK ;

Spingler, B ;

Tsien, RY ;

Lippard, SJ .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (32) :7831-7841

[4] Crystal structure of human serum albumin complexed with fatty acid reveals an asymmetric distribution of binding sites [J].

Curry, S ;

Mandelkow, H ;

Brick, P ;

Franks, N .

NATURE STRUCTURAL BIOLOGY, 1998, 5 (09) :827-835

[5] Signaling recognition events with fluorescent sensors and switches [J].

de Silva, AP ;

Gunaratne, HQN ;

Gunnlaugsson, T ;

Huxley, AJM ;

McCoy, CP ;

Rademacher, JT ;

Rice, TE .

CHEMICAL REVIEWS, 1997, 97 (05) :1515-1566

[6] ROLE OF ALBUMIN IN HUMAN PHYSIOLOGY AND PATHOPHYSIOLOGY [J].

DOWEIKO, JP ;

NOMPLEGGI, DJ .

JOURNAL OF PARENTERAL AND ENTERAL NUTRITION, 1991, 15 (02) :207-211

[7] A GENERAL, WIDE-RANGE SPECTROFLUOROMETRIC METHOD FOR MEASURING THE SITE-SPECIFIC AFFINITIES OF DRUGS TOWARD HUMAN SERUM-ALBUMIN [J].

EPPS, DE ;

RAUB, TJ ;

KEZDY, FJ .

ANALYTICAL BIOCHEMISTRY, 1995, 227 (02) :342-350

[8] The binding of novel two-color fluorescence probe FA to serum albumins of different species [J].

Ercelen, S ;

Klymchenko, AS ;

Mély, Y ;

Demchenko, AP .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2005, 35 (05) :231-242

[9] Interactions of lactone, carboxylate and self-aggregated forms of camptothecin with human and bovine serum albumins [J].

Fleury, F ;

Kudelina, I ;

Nabiev, I .

FEBS LETTERS, 1997, 406 (1-2) :151-156

[10] Structure, dynamics, and stability variation in bacterial albumin binding modules: Implications for species specificity [J].

He, Yanan ;

Rozak, David A. ;

Sari, Nese ;

Chen, Yihong ;

Bryan, Philip ;

Orban, John .

BIOCHEMISTRY, 2006, 45 (33) :10102-10109