Phytase expression in transgenic soybeans: stable transformation with a vector-less construct

被引：33

作者：

Gao, Xiao Rong ^{[1
]}

Wang, Guo Kun ^{[1
]}

Su, Qiao ^{[1
]}

Wang, Yan ^{[1
]}

An, Li Jia ^{[1
]}

机构：

[1] Dalian Univ Technol, Dept Biosci & Biotechnol, Dalian 116024, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 11期

关键词：

pollen tube pathway transformation; linear DNA; phytase gene; transgenic soybean;

D O I：

10.1007/s10529-007-9439-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A minimal linear gene cassette (35S-phytase gene-nos) with T-DNA borders was acquired by PCR and directly introduced into soybean through the pollen tube pathway. A total of 13% of T-1 plants were positive for phyA by specific PCR. Southern blot analyses showed that phyA insertions were harbored stably in T-2 progeny. Phytase expression level increased 2.5-fold over a 6-week period; its highest activity was 150 U/mg protein, compared to 56 U/mg protein in untransformed controls. Activity of phytase increased to 125 FTU/kg in T-3 transgenic seeds as compared to 64 FTU/kg in wild-type plants.

引用

页码：1781 / 1787

页数：7