Imaging of Ca2+ intracellular dynamics with a third-harmonic generation microscope

被引：37

作者：

Canioni, L

Rivet, S

Sarger, L

Barille, R

Vacher, P

Voisin, P

机构：

[1] CNRS, UMR 5798, Ctr Phys Mol & Ondes Hertziennes, F-33405 Talence, France

[2] CNRS, UMR 5543, Lab Physiol & Physiopathol Signalisat Cellulaire, F-33076 Bordeaux, France

[3] CNRS, UMR 146, Lab Resonance Magnet Syst Biol, F-33076 Bordeaux, France

来源：

OPTICS LETTERS | 2001年 / 26卷 / 08期

关键词：

D O I：

10.1364/OL.26.000515

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We describe the promising development of third-harmonic generation (THG) in laser scanning microscopy for study of the functional imaging of live biological cells. The dynamics of Ca2+ in biological cells is shown. The Ca2+ signal consists of a transient increase in the intracellular concentration. THG microscopy allows one to temporally visualize the release of Ca2+ from internal stores and (or) calcium influx. (C) 2001 Optical Society of America.

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页码：515 / 517

页数：3

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